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Fig. 4 | Biotechnology for Biofuels

Fig. 4

From: Exploring fatty alcohol-producing capability of Yarrowia lipolytica

Fig. 4

Effect of Tafar1 expression strength on fatty alcohol-producing capability. Δfao1 strains expressing Tafar1 of different copy numbers were used for effect evaluation of Tafar1 expression strength on fatty alcohol-producing capability. Low-copy CEN plasmid (~ 1.6 copies/cell) was utilized to control the Tafar1 copy number (episomal expression, Epi) by manipulating Tafar1 expression cassette numbers. After transformation of plasmid with different numbers of Tafar1 expression cassette into Δfao1 or Tafar1-2copy-Δfao1 strains, transformants were obtained (Tafar1 gene copy number = 1.6 × N + M, where N is Tafar1 expression cassette number within the plasmid and M is 2 or 0 for strains with or without genome integration of Tafar1 respectively) and subsequently used for inoculation into SD-LEU. Tafar1 expression levels (a) and fatty alcohol-producing rate (b) was detected after 24 h culturing. Relative expression of mRNA was normalized relative to the actin gene and the values reflect fold change expression compared to Po4f uracil + leucine + Tafar1 Epi strain. Real-time PCR results are means of two biological replicates SE. Each PCR was run three times. Results of fatty alcohol producing rate are the mean of duplicate experiments and error bars indicate standard deviations

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