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Fig. 1 | Biotechnology for Biofuels

Fig. 1

From: A markerless gene deletion and integration system for Thermoanaerobacter ethanolicus

Fig. 1

Plasmid maps for gene deletion and integration: a backbone plasmid, b plasmid for tdk deletion in wild-type, c plasmid for ldh deletion in stain X20, d plasmid for tdk integration in strain X20. Red: primers; green: promoters; yellow: coding region or origin of replication; blue: coding region for htk; gray: region for homologous recombination. AP ampicillin; up upstream; down downstream; ori origin; R resistance; P promoter; red arrow primer direction

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