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Table 2 List of primers used

From: A markerless gene deletion and integration system for Thermoanaerobacter ethanolicus

Name

Sequence

p1

GAGAAAGGCGGACAGGTA

p2

AATTCTCTTACTGTCATGCC

p3

TGCCTCCTCATCCTCTTC

p4, p14

TTAACCTATAAAAATAGGCGTATCACGAGATGCATCAGCGCCCTGAAGAAGTAACTGACA

p5

CCACCTATATCGGTTTTCTTCATCTCTACACCTCTTTTAGTCTTCACCACTCTAACCCCC

p6

AAAAGAGGTGTAGAGATGAAGAAAACC

p7, p17

CTCCCCGCGCGTTGGCCGATTCATTAATGATGCATCAGGCAGTTCCGCTTCAAGTTTAGG

p8

GACATTAACCTATAAAAATAGGCGTATCACGAGATGCATCAGTGCCGCCTTGTACAGTTT

p9

TTTTCACACTGTGACTTTTTATATGCAAAAAAGAGGGTTTCCACCGACAAATCCAGAGCC

p10

GGAAACCCTCTTTTTTGCAT

p11

ATCTTACCTATCACCTCAAATGGTTCGCTGGGTTTTACGCAGCGAACTTCCCGTAGCAAT

p12

CTAATCTTTTCTGAAGTACATCCGCAACTGTCCATACTCCAGTTGATATAAGCCACGGGG

p13

CCGCGCGTTGGCCGATTCATTAATGATGCATCAGCCTCTTATATGTCAAGCTCTTGTATT

p15

TACCTATCACCTCAAATGGTTCGCTGGGTTTTACGCAGGCAGTTCCGCTTCAAGTTTAGG

p16

TCTGAAGTACATCCGCAACTGTCCATACTCCAGAAAAGAGGTGTAGAGATGAAGAAAACC

p18

AGAAGAATTGGAGGCCAT

p19

CTGCAGAATAAGTAAGGCT

p20

TGCCGCCTTGTACAGTTT (binding sites the same as p8)

p21

CGAACTTCCCGTAGCAAT (binding sites the same as p11)

  1. Note: primer-binding regions are highlighted in italic fonts