Fig. 6

Overexpression of an miR156-resistant rPvSPL2 in switchgrass. a Morphological characterization of miR156-, rPvSPL2-, and rPvSPL2 miR156-overexpressing transgenic switchgrass plants. Control plant was produced with pANIC6D empty vector from the same batch of experiment. b Tiller number of rPvSPL2-overexpressing transgenic switchgrass lines. c Expression levels of PvSPL2 in rPvSPL2-overexpressing transgenic switchgrass lines were revealed by qRT-PCR. Switchgrass Ubq2 was used as the reference for normalization. PvSPL2: sum of exo- and endo-PvSPL2 transcript versions. d Tiller number of rPvSPL2 miR156-overexpressing transgenic lines. e Expression levels of PvSPL2 in rPvSPL2 miR156-overexpressing transgenic lines were revealed by qRT-PCR. Switchgrass Ubq2 was used as the reference for normalization. PvSPL2: sum of exo- and endo-PvSPL2 transcript versions. f Expression levels of mature miR156 in rPvSPL2 miR156-overexpressing transgenic lines were detected and quantified by a highly sensitive quantitative real-time PCR method. miRNA168 was used as the reference for normalization. Values are mean ± SE (n = 3)