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Fig. 4 | Biotechnology for Biofuels

Fig. 4

From: A water-forming NADH oxidase regulates metabolism in anaerobic fermentation

Fig. 4

qRT-PCR analysis of S. cerevisiae and C. acetobutylicum strains. Cycle threshold (Ct) values from three technical replicates were averaged. Fold differences were calculated using the reference gene ACT1 for S. cerevisiae strains the housekeeping gene CA_C0279 for C. acetobutylicum strains. a Results of qRT-PCR for genes related to ethanol and glycerol metabolism in S. cerevisiae. noxE NADH oxidase, ALD3 aldehyde dehydrogenase, ALD6 aldehyde dehydrogenase, ADH1 alcohol dehydrogenase, GPD1 glycerol-3-phosphate dehydrogenase, GUT1 glycerol kinase, TDH2 glyceraldehyde-3-phosphate dehydrogenase, HXT6 hexose transporter. b Results of qRT-PCR for genes related in ABE and acid metabolism in C. acetobutylicum. noxE NADH oxidase, Pta phosphotransacetylase, AskA acetate kinase, ctfA butyrate-acetoacetate CoA-transferase, Adc acetoacetate decarboxylase, Buk butyrate kinase, Ptb phosphate butyryltransferase, adhE bifunctional acetaldehyde-CoA/alcohol dehydrogenase, hydA hydrogen dehydrogenase

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