The two Rasamsonia emersonii α-glucuronidases, ReGH67 and ReGH115, show a different mode-of-action towards glucuronoxylan and glucuronoxylo-oligosaccharides

Table 1 Performance of GH115 α-glucuronidases described in literature towards polymeric and oligomeric xylan substrates

α-glucuronidases	ScAgu115	PsAgu115	SpAgu115	BoAgu115	ReGH115
Organism	Schizophyllum commune	Pichia stipitis	Streptomyces pristinaespiralis	Bacteroides ovatus	Rasamsonia emersonii
Division	Basidiomycete	Ascomycete	Actinobacterium	Bacteroidete	Ascomycete
Mw (Kda)	125	120	nt	199	150
pH optimum	nt	4.4	nt	nt	4.0
T optimum (°C)	nt	60	nt	nt	65-70
3D-structure	No	No	No	Yes	No
Activity on xylan
BeWX	nt	+	+	nt	+
BiWX	nt	nt	+	nt	+
SpW	+	nt	nt	nt	nt
WWX	nt	nt	nt	+	nt
Activity on U^4m2X ^a_n
U^4m2	nt	nt	nt	+	+
U^4m2X	nt	nt	nt	+	+
XU^4m2	+	+	nt	nt	+
U^4m2XX	+	+	nt	+	+
XU^4m2X	++	+	nt	++	+
XXU^4m2	nt	nt	nt	nt	+
U^4m2XXX	nt	nt	nt	++	nt
XU^4m2XX	++	+	nt	nt	nt
XXU^4m2X	nt	nt	+	nt	+
XXU^4m2XX	++	+	nt	nt	nt

Active towards substrate (+), preferential degradation (++)
nt Not tested, BeWX Beechwood xylan, BiWX Birchwood xylan, SpW Spruce wood, WWX Willow wood xylan
ScAgu115 Chong et al. [16]; Tenkanen and Siika-aho [15]; PsAgu115 Ryabova et al. [14]; Kolenová et al. [23]; SpAgu115 Fujimoto et al. [12]; BoAgu115 Rogowski et al. [13]
^aNomenclature used is according to Faure et al. [21]

ISSN: 2731-3654