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Table 2 Single reaction monitoring (SRM) settings of AAc analysed by UHPLC-MS

From: The two Rasamsonia emersonii α-glucuronidases, ReGH67 and ReGH115, show a different mode-of-action towards glucuronoxylan and glucuronoxylo-oligosaccharides

Segment 1 (0–16 min) Segment 2 (16–50 min)
Scan event name Parent mass (m/z) Main MS2 fragment (F) Rt (min) Scan event name Parent mass (m/z) Main MS2 fragment (F) Rt (min)
F1 (m/z) F2 (m/z) F1 (m/z) F2 (m/z)
TIC 50–1000 na na na TIC 50–1000 na na na
SRM 1 856 666 780 14 SRM 1 460 270 384 16.2
SRM 2 724 533 648 14.7 SRM 2 724 534 648 17.5
SRM 2 724 533 648 15.3 SRM 3 592 402 516 17.5
SRM 3 na na na na SRM 3 592 402 516 21.2
  1. Rt retention time in minutes, na not applicable