Fig. 5

Graphs 1, 2, and 3 represent the total glucan + xylan yield ranks for 22 variants from at different processing conditions. The highest yield is rank as 1, and the lowest yield is ranked as 22. Conditions from left to right are (1) enzymatic hydrolysis for 120 h without prior pretreatment, (2) HTPH at 140 °C for 264.4 min (logR₀ = 3.6), (3) HTPH at 160 °C for 68.1 min (logR₀ = 3.6), (4) HTPH at 180 °C for 17.6 min (logR₀ = 3.6), and (5) HTPH at 180 °C for 44.1 min (logR₀ = 4.0). Enzymatic hydrolysis for all HTPH experiments was applied for 24 h. The enzyme loading for all conditions was 75 mg cellulase protein + 25 mg xylanase protein per gram glucan + xylan in the raw biomass