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Table 1 Hydrothermal pretreatment conditions applied to poplar variants

From: Natural genetic variability reduces recalcitrance in poplar

Condition representative symbol Condition name Pretreatment severity factor (log10R0)a Temperature (°C) Time (minutes) Co-hydrolysis enzyme loading Hydrolysis time (hours)
A No pretreatment, only enzymatic hydrolysis N/A N/A N/A 75 mg cellulase protein + 25 mg xylanase protein per gram glucan + xylan in raw biomass. Assuming 66.6 % glucan + xylan in biomass 120
B HTPH 140 °C logR0 = 3.6 3.6 140 264.4 24
C HTPH 160 °C logR0 = 3.6 3.6 160 68.1 24
D HTPH 180 °C logR0 = 3.6 3.6 180 17.6 24
E HTPH 180 °C logR0 = 4.0 4.0 180 44.1 24
  1. a R0 = t*exp[(T-100)/14.75)], where t is the time in minutes and T is the temperature in  °C. N/A not applicable. log10R0 is called the pretreatment severity factor [17]