Fig. 5

Wide-field (grey) and dSTORM super resolution (magenta) fluorescent microscopy images of CipC-expressing C. acetobutylicum bacteria. The bacteria were visualised by their autofluorescence, detected in the green channel and shown here in grey; CipC was labelled by addition of anti-FLAG mouse monoclonal antibody followed by anti-mouse goat antibody conjugated to Alexa Fluor 647 dye and is shown as magenta pixels detected as single molecules in the far red channel. a Negative control (CEL07, overexpressing the native srtA gene) showing few background events, see the few pixels in magenta. b C. acetobutylicum CEL08 bacteria (producing CipC2F3-CA_0205ss and overexpressing the native srtA gene) displaying high number of CipC molecules on their surface. c Single CEL08 bacterium; the magenta pixels represent single molecule localisations of the labelled CipC. Scale bars a, b 2 µm, c 0.5 µm