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Fig. 4 | Biotechnology for Biofuels

Fig. 4

From: DNA microarray of global transcription factor mutant reveals membrane-related proteins involved in n-butanol tolerance in Escherichia coli

Fig. 4

Colony-forming efficiency of E. coli knockout strains on LB agar. Seven single-gene knockouts were cultured in LB medium, and 0.8 % n-butanol was added at OD660 0.8 followed by incubation for 1.5 h. Then, cell cultures were serially diluted at tenfold gradient, and 10 μL of the diluted solution was spotted onto LB agar for incubation at 37 °C overnight. E. coli JM109 was used as the control

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