DNA microarray of global transcription factor mutant reveals membrane-related proteins involved in n-butanol tolerance in Escherichia coli

Table 2 Comparison of fatty acid composition of knockouts and control

Major fatty acids	Fatty acid (%)^a			Difference △yibT	p value^b	Difference △yghW	p value^b
Major fatty acids	JM109	△yibT	△yghW	Difference △yibT	p value^b	Difference △yghW	p value^b
C11:0	0.20 ± 0.04	0.00 ± 0.00	0.13 ± 0.04	−0.20	***	−0.08	**
C12:0	0.69 ± 0.44	0.33 ± 0.04	0.45 ± 0.12	−0.36	*	−0.25	*
C13:0	0.00 ± 0.00	0.05 ± 0.07	0.07 ± 0.09	0.05	*	0.07	*
C14:0	2.24 ± 0.96	1.42 ± 0.27	1.41 ± 0.13	−0.82	*	−0.84	*
C15:0	0.65 ± 0.52	2.45 ± 0.05	3.03 ± 0.87	1.80	***	2.38	***
C15:1	0.00 ± 0.00	0.12 ± 0.16	0.31 ± 0.07	0.12	*	0.31	***
C16:0	26.96 ± 1.32	18.86 ± 0.33	15.48 ± 1.18	−8.10	***	−11.49	***
C16:1	9.06 ± 1.08	10.90 ± 1.05	11.08 ± 1.52	1.84	**	2.02	*
C17:0	1.52 ± 0.42	6.18 ± 0.77	5.67 ± 0.37	4.66	***	4.15	***
C17:1	4.69 ± 0.62	4.80 ± 0.39	6.35 ± 0.88	0.11	*	1.66	**
C18:0	9.56 ± 0.95	6.49 ± 1.44	5.36 ± 0.31	−3.07	**	−4.20	***
C18:1	21.03 ± 1.36	25.23 ± 0.73	27.05 ± 0.37	4.20	***	6.02	***
C18:2	0.03 ± 0.04	0.06 ± 0.08	0.42 ± 0.29	0.03	*	0.39	**
C18:3	0.38 ± 0.54	0.00 ± 0.00	0.04 ± 0.06	−0.38	*	−0.34	*
C19:1	1.22 ± 0.17	0.08 ± 0.11	0.07 ± 0.09	−1.14	***	−1.16	***
C20:0	0.23 ± 0.01	0.08 ± 0.11	0.16 ± 0.06	−0.15	**	−0.08	*
C20:1	1.07 ± 0.62	0.00 ± 0.00	0.00 ± 0.00	−1.07	**	−1.07	**
C22:1	0.13 ± 0.18	0.06 ± 0.08	0.05 ± 0.07	−0.07	*	−0.08	*
C25:0	0.06 ± 0.08	0.07 ± 0.09	0.00 ± 0.00	0.01	*	−0.06	*
SFA	42.09 ± 0.68	35.91 ± 0.08	31.73 ± 1.92	−6.18	***	−10.37	***
UFA	37.59 ± 0.51	41.22 ± 2.28	45.36 ± 3.34	3.63	**	7.76	***

^aThe values represent percentages of total fatty acids and are means ± standard deviations from three independent experiments
^bStatistical significance between knockout and control strain (E.coli JM109)
* p > 0.05, ** 0.01 < p < 0.05, *** p < 0.01

ISSN: 2731-3654