Skip to main content

Table 2 Comparison of fatty acid composition of knockouts and control

From: DNA microarray of global transcription factor mutant reveals membrane-related proteins involved in n-butanol tolerance in Escherichia coli

Major fatty acids Fatty acid (%)a Difference yibT p valueb Difference yghW p valueb
JM109 yibT yghW
C11:0 0.20 ± 0.04 0.00 ± 0.00 0.13 ± 0.04 −0.20 *** −0.08 **
C12:0 0.69 ± 0.44 0.33 ± 0.04 0.45 ± 0.12 −0.36 * −0.25 *
C13:0 0.00 ± 0.00 0.05 ± 0.07 0.07 ± 0.09 0.05 * 0.07 *
C14:0 2.24 ± 0.96 1.42 ± 0.27 1.41 ± 0.13 −0.82 * −0.84 *
C15:0 0.65 ± 0.52 2.45 ± 0.05 3.03 ± 0.87 1.80 *** 2.38 ***
C15:1 0.00 ± 0.00 0.12 ± 0.16 0.31 ± 0.07 0.12 * 0.31 ***
C16:0 26.96 ± 1.32 18.86 ± 0.33 15.48 ± 1.18 −8.10 *** −11.49 ***
C16:1 9.06 ± 1.08 10.90 ± 1.05 11.08 ± 1.52 1.84 ** 2.02 *
C17:0 1.52 ± 0.42 6.18 ± 0.77 5.67 ± 0.37 4.66 *** 4.15 ***
C17:1 4.69 ± 0.62 4.80 ± 0.39 6.35 ± 0.88 0.11 * 1.66 **
C18:0 9.56 ± 0.95 6.49 ± 1.44 5.36 ± 0.31 −3.07 ** −4.20 ***
C18:1 21.03 ± 1.36 25.23 ± 0.73 27.05 ± 0.37 4.20 *** 6.02 ***
C18:2 0.03 ± 0.04 0.06 ± 0.08 0.42 ± 0.29 0.03 * 0.39 **
C18:3 0.38 ± 0.54 0.00 ± 0.00 0.04 ± 0.06 −0.38 * −0.34 *
C19:1 1.22 ± 0.17 0.08 ± 0.11 0.07 ± 0.09 −1.14 *** −1.16 ***
C20:0 0.23 ± 0.01 0.08 ± 0.11 0.16 ± 0.06 −0.15 ** −0.08 *
C20:1 1.07 ± 0.62 0.00 ± 0.00 0.00 ± 0.00 −1.07 ** −1.07 **
C22:1 0.13 ± 0.18 0.06 ± 0.08 0.05 ± 0.07 −0.07 * −0.08 *
C25:0 0.06 ± 0.08 0.07 ± 0.09 0.00 ± 0.00 0.01 * −0.06 *
SFA 42.09 ± 0.68 35.91 ± 0.08 31.73 ± 1.92 −6.18 *** −10.37 ***
UFA 37.59 ± 0.51 41.22 ± 2.28 45.36 ± 3.34 3.63 ** 7.76 ***
  1. aThe values represent percentages of total fatty acids and are means ± standard deviations from three independent experiments
  2. bStatistical significance between knockout and control strain (E.coli JM109)
  3. p > 0.05, ** 0.01 < p < 0.05, *** p < 0.01