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Table 3 Enzyme activity comparison

From: Simultaneous achievement of high ethanol yield and titer in Clostridium thermocellum

Strain name

PFK activitya

ALDH activity

ADH activity

GAPDH activity

PPi

NADH

NADPH

NADH

NADPH

NADH

Wild type

1.60 ± 0.40b

N/A

N/A

12.53 ± 2.59

N/A

0.38 ± 0.09

AG553

2.31 ± 0.54

1.32 ± 0.40

0.00 ± 0.06c

7.67 ± 1.19

6.82 ± 0.16

0.28 ± 0.07

AG601

2.84 ± 0.37

1.10 ± 0.17

0.00 ± 0.14

8.63 ± 0.74

6.74 ± 0.57

0.40 ± 0.11

LL1210

4.45 ± 0.81

2.92 ± 0.89

0.02 ± 0.11

14.88 ± 2.33

14.9 ± 0.31

0.53 ± 0.15

  1. aActivity units are in U/mg protein
  2. bMeasured in triplicates with same sample at different concentrations. Error represents one standard deviation, n = 3
  3. cWhen background activity was higher than reaction activity, the values are reported as zero
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Biotechnology for Biofuels and Bioproducts

ISSN: 2731-3654

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