Skip to main content

Table 3 Enzyme activity comparison

From: Simultaneous achievement of high ethanol yield and titer in Clostridium thermocellum

Strain name PFK activitya ALDH activity ADH activity GAPDH activity
PPi NADH NADPH NADH NADPH NADH
Wild type 1.60 ± 0.40b N/A N/A 12.53 ± 2.59 N/A 0.38 ± 0.09
AG553 2.31 ± 0.54 1.32 ± 0.40 0.00 ± 0.06c 7.67 ± 1.19 6.82 ± 0.16 0.28 ± 0.07
AG601 2.84 ± 0.37 1.10 ± 0.17 0.00 ± 0.14 8.63 ± 0.74 6.74 ± 0.57 0.40 ± 0.11
LL1210 4.45 ± 0.81 2.92 ± 0.89 0.02 ± 0.11 14.88 ± 2.33 14.9 ± 0.31 0.53 ± 0.15
  1. aActivity units are in U/mg protein
  2. bMeasured in triplicates with same sample at different concentrations. Error represents one standard deviation, n = 3
  3. cWhen background activity was higher than reaction activity, the values are reported as zero