Table 5 Proteins identified by MS in different bands in SDS-PAGE and different enzyme activities measured by MUC and CMC zymograms
Lines | Protein identified by MS | PSMsa | Possible main proteins in band | Enzyme activity detected by CMC | Enzyme activity detected by MUC |
|---|---|---|---|---|---|
1 | − | – | BGL1 | − | + |
2 | Cel5C | 15 | Cel5C, Cel5B | + | − |
Cel5B | 11 | + | − | ||
3 | Cel7A-2 | 446 | Cel7A-2 | − | + |
Cel7B | 69 | + | − | ||
cel7A-1 | 45 | − | + | ||
SWO | 40 | − | − | ||
Cel5C | 30 | + | − | ||
4 | Cel7A-2 | 348 | Cel7A-2, Cel5B | − | + |
Cel5B | 111 | + | − | ||
5 | Cel6A | 402 | Cel6A, Cel5B | − | − |
Cel5B | 157 | + | − | ||
Cel7A-1 | 79 | − | + | ||
Cel7A-2 | 60 | − | + | ||
6 | Xylanase10A | 897 | Xylanase10A | + | − |
7 | Cel6A | 1179 | Cel6A | − | − |
Xylanase10A | 96 | − | + | ||
8 | Cel7A-2 | 423 | Cel7A-2 | − | + |
Chi18A | 101 | − | − | ||
Cel6A | 91 | − | − | ||
Xylanase10A | 78 | + | − | ||
Cel5B | 56 | + | − | ||
9 | Xylanase10B | 1257 | Xylanase10B | + | − |
Cel7A-2 | 190 | − | + | ||
Abf62A | 166 | − | − | ||
10 | Cel61A | 364 | Cel61A, Cel6A | + | − |
Cel6A | 283 | − | − | ||
Fae1A | 136 | − | − |