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Table 5 Proteins identified by MS in different bands in SDS-PAGE and different enzyme activities measured by MUC and CMC zymograms

From: Adsorption and mechanism of cellulase enzymes onto lignin isolated from corn stover pretreated with liquid hot water

Lines Protein identified by MS PSMsa Possible main proteins in band Enzyme activity detected by CMC Enzyme activity detected by MUC
1 BGL1 +
2 Cel5C 15 Cel5C, Cel5B +
Cel5B 11 +
3 Cel7A-2 446 Cel7A-2 +
Cel7B 69 +
cel7A-1 45 +
SWO 40
Cel5C 30 +
4 Cel7A-2 348 Cel7A-2, Cel5B +
Cel5B 111 +
5 Cel6A 402 Cel6A, Cel5B
Cel5B 157 +
Cel7A-1 79 +
Cel7A-2 60 +
6 Xylanase10A 897 Xylanase10A +
7 Cel6A 1179 Cel6A
Xylanase10A 96 +
8 Cel7A-2 423 Cel7A-2 +
Chi18A 101
Cel6A 91
Xylanase10A 78 +
Cel5B 56 +
9 Xylanase10B 1257 Xylanase10B +
Cel7A-2 190 +
Abf62A 166
10 Cel61A 364 Cel61A, Cel6A +
Cel6A 283
Fae1A 136
  1. “+” special activity detected in the enzyme, “−” no activity detected
  2. aPSMs indicate the number of peptide fragments detected for the protein analyzed by mass spectrum