Table 1 Strains used in present study
From: Strain and bioprocess improvement of a thermophilic anaerobe for the production of ethanol from wood
Strain # | Description of genetic manipulation | Genotype |
|---|---|---|
M355 | Markerless KO of genes for acetate and lactate production | pta/ack(−) L-ldh(−) |
M795–M809 | Mix of strains generated by Panlabs using NTG mutagenesis and selection on wood hydrolysate | pta/ack(−) L-ldh(−) |
M863 | Selection in Cytostat with synthetic mix of inhibitors and 20 g/L ethanol | pta/ack(−) L-ldh(−) |
M1151 | Addition of urease, fix of metE and markerless KO of Tsac_0795 | pta/ack(−) L-ldh(−) Tsac_0795(−) urease(+) metE(+) |
M1291 | Markerless KO of putative EPS operon (phosphoglucomutase, UDP-G1P transferase, transmembrane protein, near gene Tsac_1471) | pta/ack(−) L-ldh(−) Tsac_0795(−) urease(+) metE(+) EPSoperon(−) |
M1442a | Selection in auxostat for fast growth in glucose, xylose, arabinose, and acetic acid | pta/ack(−) L-ldh(−) Tsac_0795(−) urease(+) metE(+) EPSoperon(−) |
M2476 | Markerless KO of perR | pta/ack(−) L-ldh(−) Tsac_0795(−) urease(+) metE(+) EPSoperon(−) perR(−) |
M2886 | Insertion of pta/ack-KanR into methylglyoxal synthase mgs | pta/ack(−) L-ldh(−) Tsac_0795(−) urease(+) metE(+) EPSoperon(−) perR(−) mgs::pta/ack-KanR |