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Table 1 Strains used in present study

From: Strain and bioprocess improvement of a thermophilic anaerobe for the production of ethanol from wood

Strain # Description of genetic manipulation Genotype
M355 Markerless KO of genes for acetate and lactate production pta/ack(−) L-ldh(−)
M795–M809 Mix of strains generated by Panlabs using NTG mutagenesis and selection on wood hydrolysate pta/ack(−) L-ldh(−)
M863 Selection in Cytostat with synthetic mix of inhibitors and 20 g/L ethanol pta/ack(−) L-ldh(−)
M1151 Addition of urease, fix of metE and markerless KO of Tsac_0795 pta/ack(−) L-ldh(−) Tsac_0795(−) urease(+) metE(+)
M1291 Markerless KO of putative EPS operon (phosphoglucomutase, UDP-G1P transferase, transmembrane protein, near gene Tsac_1471) pta/ack(−) L-ldh(−) Tsac_0795(−) urease(+) metE(+) EPSoperon(−)
M1442a Selection in auxostat for fast growth in glucose, xylose, arabinose, and acetic acid pta/ack(−) L-ldh(−) Tsac_0795(−) urease(+) metE(+) EPSoperon(−)
M2476 Markerless KO of perR pta/ack(−) L-ldh(−) Tsac_0795(−) urease(+) metE(+) EPSoperon(−) perR(−)
M2886 Insertion of pta/ack-KanR into methylglyoxal synthase mgs pta/ack(−) L-ldh(−) Tsac_0795(−) urease(+) metE(+) EPSoperon(−) perR(−) mgs::pta/ack-KanR
  1. aStrain M1442 is also known as LL1049