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Table 4 Genomic resequencing results from strains in the present study: large-scale sequence variations and their occurrence in each strain

From: Strain and bioprocess improvement of a thermophilic anaerobe for the production of ethanol from wood

Locus

Nucleotides

Description

LL1025 (WT)

M863

M1442

M2886

Tsac_0179

 

Engineered ldh deletion

WT

Deletion

Deletion

Deletion

Tsac_0389

424,393–424,493

Small deletion in CRISPR repeat region

WT

Deletion

Deletion

Deletion

Tsac_0832

875,581–875,753

Small deletion in intergenic repeat region

WT

Deletion

Deletion

Deletion

Tsac_1744–1745

 

Engineered pta/ack deletion

WT

Deletion

Deletion

Deletion

Tsac_2564

2618,783–2618,797

Transposon insertion in putative promoter of gene for PTS IIA subunit

WT

Putative transposon

Putative transposon

Putative transposon

Tsac_0795

 

Engineered deletion

WT

WT

Deletion

Deletion

Tsac_1474–1477

 

Engineered deletion of EPS gene cluster

WT

WT

Deletion

Deletion

Tsac_2114

 

Engineered mgs deletion/insertion

WT

WT

WT

Breakpoints in 23 % of reads b

Tsac_2491

 

Engineered perR deletion

WT

WT

WT

Deletion

  1. aThe pta/ack genes were re-introduced elsewhere in the genome
  2. bThe fraction of the reads supporting the mutation (left and right breakpoints averaged). This value was >90 % for all other breakpoints