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Table 6 Fermentation data comparing strains ALK2, M1442, and M2886 in pH-controlled bioreactors in SSCF or SHF process configurations

From: Strain and bioprocess improvement of a thermophilic anaerobe for the production of ethanol from wood

Substrate Mock hydrolysate Mock hydrolysate Mock hydrolysate Pre-treated hardwood Pre-treated hardwood Pre-treated hardwood Hardwood hydrolysate
Process type SSCF SSCF SSCF SSCF SSCF SSCF SHF
Fermentation volume (liters) 1 1 8 1 1 100 1
Strain ALK2 M1442 M1442 ALK2 M2886 M2886 M2886
Initial concentrations
 Solids (%) 16.5  16.5  16.5  12.0  12.0  12.0  0.0 
 Cellulose (g/L) 100 100 100 64.5 64.5 64.5 0.0
 Glucose (g/L) 20 20 20 1.0 1.0 1.2 88.6
 Xylose (g/L) 35 35 35 13.9 13.9 16.6 24.3
 Other sugars (g/L)     3.8 3.8 3.2 5.2
 Acetic acid and other inhibitors (g/L) 10.5 10.5 10.0 6.3 6.3 6.3 4.5
Fermentation performance
 Fermentation time (hours) 97 93 90 60 60 60 60
 Final ethanol titer (g/L) 45.7 61.4 54.7 32.6 32.0 30.8 49.5
 Cellulose conversion (%) 71.0  75.1  83.4  83.6  80.4  77.2  n/a
 Glucose utilization (%) 79.5  93.6  85.9  97.2  94.7  99.5  89.3 
 Xylose utilization (%) 58.1  99.6  80.9  82.3  86.7  100.0  91.6 
 Metabolic yield (%) 90.5  91.5  85.8  75.9  81.0  78.4  90.1