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Table 2 Effects of metal ions and chemical reagents on the TlGlu16A activity

From: Improvement of the thermostability and catalytic efficiency of a highly active β-glucanase from Talaromyces leycettanus JCM12802 by optimizing residual charge–charge interactions

Chemical Relative activity (%)a Chemical Relative activity (%)
5 mM 10 mM 5 mM 10 mM
None 100.0 ± 0.9 100.0 ± 0.8 Fe3+ 106.2 ± 2.1 99.6 ± 1.9
Na+ 110.8 ± 1.8 120.4 ± 1.5 Pb2+ 95.6 ± 2.7 91.8 ± 2.8
Co2+ 116.6 ± 1.8 121.2 ± 1.7 Mn2+ 87.2 ± 1.2 66.1 ± 2.1
K+ 109.3 ± 2.1 119.6 ± 2.7 Cu2+ 86.1 ± 2.4 48.5 ± 1.8
Ca2+ 108.8 ± 1.3 114.9 ± 1.3 Ag+ 78.4 ± 1.9 1.2 ± 0.7
Ni2+ 108.3 ± 1.6 117.7 ± 2.1 β-Mercaptoethanol 130.8 ± 3.0 146.3 ± 2.4
Mg2+ 106.5 ± 1.7 112.0 ± 2.1 EDTA 110.3 ± 2.5 120.0 ± 2.1
Cr3+ 103.1 ± 1.6 116.0 ± 1.2 SDS 64.7 ± 3.1 64.6 ± 1.0
Zn2+ 101.4 ± 2.1 111.3 ± 1.8    
  1. aValues represent mean ± SD (n = 3) relative to the untreated control samples