Improvement of the thermostability and catalytic efficiency of a highly active β-glucanase from Talaromyces leycettanus JCM12802 by optimizing residual charge–charge interactions

Table 2 Effects of metal ions and chemical reagents on the TlGlu16A activity

Chemical	Relative activity (%)^a		Chemical	Relative activity (%)
Chemical	5 mM	10 mM	Chemical	5 mM	10 mM
None	100.0 ± 0.9	100.0 ± 0.8	Fe³⁺	106.2 ± 2.1	99.6 ± 1.9
Na⁺	110.8 ± 1.8	120.4 ± 1.5	Pb²⁺	95.6 ± 2.7	91.8 ± 2.8
Co²⁺	116.6 ± 1.8	121.2 ± 1.7	Mn²⁺	87.2 ± 1.2	66.1 ± 2.1
K⁺	109.3 ± 2.1	119.6 ± 2.7	Cu²⁺	86.1 ± 2.4	48.5 ± 1.8
Ca²⁺	108.8 ± 1.3	114.9 ± 1.3	Ag⁺	78.4 ± 1.9	1.2 ± 0.7
Ni²⁺	108.3 ± 1.6	117.7 ± 2.1	β-Mercaptoethanol	130.8 ± 3.0	146.3 ± 2.4
Mg²⁺	106.5 ± 1.7	112.0 ± 2.1	EDTA	110.3 ± 2.5	120.0 ± 2.1
Cr³⁺	103.1 ± 1.6	116.0 ± 1.2	SDS	64.7 ± 3.1	64.6 ± 1.0
Zn²⁺	101.4 ± 2.1	111.3 ± 1.8

ISSN: 2731-3654