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Table 2 Kinetics of hydrolysis of aryl-monosaccharides by THSAbf

From: The GH51 α-l-arabinofuranosidase from Paenibacillus sp. THS1 is multifunctional, hydrolyzing main-chain and side-chain glycosidic bonds in heteroxylans

Substrate K M (mM) k cat (s−1) k cat/K M (s−1 mM−1) SA (U mg−1)
pNP-Araf 0.31 ± 0.1 328 ± 23 1050 426 ± 36
pNP-Xylp 23.8 ± 4.1a 10.6 ± 0.9 0.44 1.70 ± 5
pNP-Galf 8.6 ± 0.9a 327 ± 20 38 103 ± 2
  1. aThese values are subject to caution since the maximum substrate concentration used in each case was less than 2 × K M. n = 3 and SD values are shown