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Table 2 Kinetics of hydrolysis of aryl-monosaccharides by THSAbf

From: The GH51 α-l-arabinofuranosidase from Paenibacillus sp. THS1 is multifunctional, hydrolyzing main-chain and side-chain glycosidic bonds in heteroxylans

Substrate

K M (mM)

k cat (s−1)

k cat/K M (s−1 mM−1)

SA (U mg−1)

pNP-Araf

0.31 ± 0.1

328 ± 23

1050

426 ± 36

pNP-Xylp

23.8 ± 4.1a

10.6 ± 0.9

0.44

1.70 ± 5

pNP-Galf

8.6 ± 0.9a

327 ± 20

38

103 ± 2

  1. aThese values are subject to caution since the maximum substrate concentration used in each case was less than 2 × K M. n = 3 and SD values are shown