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Fig. 1 | Biotechnology for Biofuels

Fig. 1

From: Engineering yeast with bifunctional minicellulosome and cellodextrin pathway for co-utilization of cellulose-mixed sugars

Fig. 1

Strategy for engineering S. cerevisiae EBY100 to co-ferment cellulose and galactose. Endoglucanase, exoglucanase and miniscaffoldin I* were expressed as fusions to an N-terminal peptide encoding the S. cerevisiae α-factor secretion signal, so that they were first secreted in culture medium and then assembled extracellularly through the interactions between cohesins and dockerins. β-glucosidase and cellodextrin transporter are localized in cytoplasm and cell membrane, respectively

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