Fig. 4

Screening of cellulases and determination of the enzyme assembly efficiency. Comparison of endoglucanases (a), exo-glucanases (b), and enzyme combinations (c) in various engineered yeast strains. d Analysis of the enzyme assembly efficiency by FACS with double-antibody staining. Control in (b) lacks both endoglucanase and exoglucanase. Label of X-axis in (d) indicates the fusion position of His-tag (HHHHHH) in three different EBY100 (CohII = 1, cdt-1, gh1-1, engy, cbhb). His-ScaII, His-EngY and His-cbhB mean the His-tag-fused miniscaffoldin II (ScaII, CohII = 1, C-terminus), His-tag-fused EngY (N-terminus), and His-tag-fused cbhB (N-terminus)