Fig. 5

Optimization of minicellulosome assembly and cellulosic ethanol production. a Effects of galactose concentration on miniscaffoldin II display and cell growth. The EBY100 co-expressing bifunctional minicellulosome (CohII = 4, engy, cbhb) and cellodextrin pathway (cdt-1, gh1-1) was employed. The His-tag-fused to the C-terminus of miniscaffoldin II was double-antibody stained. b Effects of cell inoculum density and miniscaffoldin II length on CMC-galactose co-fermentation. The EBY100 strains co-expressing cdt-1, gh1-1, miniscaffoldin I*, miniscaffoldin II (CohII = 1–4), engy, and cbhb were used