Fig. 8

Expression of TAG biosynthesis-associated genes in Camelina developing seeds and leaf measured by qRT-PCR. Relative combined expression of all three copies of CsWRl1, CsGPAT9, CsLPP1, CsLPP2, CsLPAT2, CsDGAT1, CsDGAT2, CsPDAT, CsWSD1, CsPDCT, CsOle1, CsOle4, and CsMGAT1. The descriptive gene names are available in supplemental tables. The bars represent the fold change in log2 scale as measured by real-time qPCR from cDNA derived from Camelina seeds at 10–15 (Cs-14) and 16–21 (Cs-21) days after flowering (DAF) and from leaf tissue (Cs-Leaf). The leaf sample was used as the calibrator for the remaining samples. Error bars represent the standard error ± SE of three biological replicates. The quantification of the genes is measured relative to the expression of the indigenous housekeeping gene β-actin