Fig. 7
From: Discovery of new cellulases from the metagenome by a metagenomics-guided strategy
a The effect of NaCl on the activity of the recombinant cel7482, cel3623 and cel36. Each purified enzyme was incubated with CMC at optimal temperature for 30 min in 50 mM citrate–phosphate buffer (pH 5.5) in the presence of 0.5–5 M NaCl. b Halotolerance of the recombinant cel7482. After pre-incubation in 0.5, 2 and 5 M NaCl for different periods of time, the residual activity was determined at 70 °C in 50 mM citrate–phosphate buffer (pH 5.5) with CMC as substrate