Table 1 Purification of the recombinant His6-tagged cel7482, cel3623 and cel36 from 500 ml of E. coli cultures using immobilized metal ion affinity chromatography
From: Discovery of new cellulases from the metagenome by a metagenomics-guided strategy
Purification step | Total protein (mg) | Total activity (Unit) | Specific activity (U/mg protein) | Yield (%) | Fold |
|---|---|---|---|---|---|
Total cell lysate | 328a, 369b, 358c | 391, 321, 229 | 1.19, 0.87, 0.64 | 100, 100, 100 | 1, 1, 1 |
Ni–NTA column | 11, 14, 13 | 316, 244, 183 | 28.73, 17.43, 14.08 | 81, 76, 80 | 24, 20, 22 |