Table 2 Kinetic parameters of β-glucosidase Bgl3A and its two mutants
Substrate | Enzyme | Specific activity (U/mg) | K i (mM) | K m (mM) | V max (U/mg) | k cat (/s) | k cat/K m (/s/mM) |
|---|---|---|---|---|---|---|---|
pNPG | Bgl3A | 905 | 14.0 | 0.18 | 1309 | 1664 | 9096 |
M1 | 1019 | 17.0 | 0.31 | 1408 | 1791 | 5778 | |
M2 | 843 | 12.3 | 0.36 | 1679 | 2136 | 4664 | |
Cellobiose | Bgl3A | 265.5 | ND | 10.4 | 618.4 | 786.0 | 75.8 |
M1 | 209.4 | ND | 8.4 | 531.6 | 676.0 | 80.5 | |
M2 | 197.6 | ND | 9.3 | 733.0 | 932.6 | 100.3 |