Fig. 3

Elucidation of function of XDH and XKS using E. coli and further studies in Y. lipolytica. a Schematic of the xylose growth challenge in E. coli using mutants lacking a native gene (xylA or xylB) in the xylose pathway and challenging E. coli to use the candidate Y. lipolytica enzyme(s) instead. b Growth curves of E. coli ΔxylB strains in the xylose challenge: E. coli ΔxylB expressing XKS (Plasmid 2) from Y. lipolytica (blue), wild-type (BW25113) positive control (white), and E. coli ΔxylB negative control (Plasmid 1) (black). c Growth curves of E. coli ΔxylA strains in the 1 % xylitol minimal media: E. coli ΔxylA expressing XYR1-XDH (Plasmid 3) from Y. lipolytica (blue), Wild type (BW25113) control (white), and E. coli ΔxylB negative control (Plasmid 1) (black). All error bars represent SD. (n = 3)