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Table 1 Strains and plasmids

From: Engineering xylose utilization in Yarrowia lipolytica by understanding its cryptic xylose pathway

  Genotype References
E. coli strains
 BW25113 rrnB T14 ΔlacZ WJ16 hsdR514 ΔaraBAD AH33 ΔrhaBAD LC78 [33, 39]
 BW25113
ΔxylA
BW25113 with xylA::kanR [33, 39]
 BW25113
ΔxylB
BW25113 with xylB::kanR [33, 39]
 BL21(DE3) fhuA2 [lon] ompT gal (λ DE3) [dcm] ∆hsdS
λ DE3 = λ sBamHIo ∆EcoRI-B int::(lacI::PlacUV5::T7 gene1) i21 ∆nin5
[40]
Y. lipolytica strains
 P01f MatA, leu2-270, ura3-302, xpr2-322, axp1-2 (ATCC no. MYA-2613) ATCC
 P01f–XKS P01f with alternate frameshift mutation in XKS gene This work
 P01f–XDH P01f with frameshift mutation in XDH gene This work
Plasmids
 1 AmpR, ColE1 ori; PlacUV5: fadD-AtfA, lacI q [4]
 2 AmpR, ColE1 ori; PlacUV5: XKS (YALI0F10923 g), lacI q This work
 3 AmpR, ColE1 ori; PlacUV5: XYR1 (YALI0D07634 g)-XDH (YALI0E12463 g), lacI q This work
 4 AmpR, ColE1 ori; PlacUV5: XYR2 (YALI0F18590 g)-XDH (YALI0E12463 g), lacI q This work
 5 AmpR, ColE1 ori; PlacUV5: XYR3 (YALI0A15906 g)-XDH (YALI0E12463 g), lacI q This work
 6 AmpR, ColE1 ori; PlacUV5: XYR4 (YALI0B21780 g)-XDH (YALI0E12463 g), lacI q This work
 7 AmpR, ColE1 ori; PlacUV5: XYR5 (YALI0C13508 g)-XDH (YALI0E12463 g), lacI q This work
 8 AmpR, ColE1 ori; PlacUV5: XYR6 (YALI0B07117 g)-XDH (YALI0E12463 g), lacI q This work
 9 AmpR, ColE1 ori; PlacUV5: XYR7 (YALI0E18348 g)-XDH (YALI0E12463 g), lacI q This work
 10 AmpR, ColE1 ori; PlacUV5: XYR8 (YALI0042092 g)-XDH (YALI0E12463 g), lacI q This work
 11 AmpR, ColE1 ori; PlacUV5: XYR9 (YALI0C09119 g)-XDH (YALI0E12463 g), lacI q This work
 12 AmpR, ColE1 ori; PlacUV5: XYR10 (YALI0F06974 g)-XDH (YALI0E12463 g), lacIq This work
 13 AmpR, ColE1 ori; PlacUV5: XYR11 (YALI0B15268 g)-XDH (YALI0E12463 g), lacI q This work
 14 AmpR, ColE1 ori; PlacUV5: XYR12 (YALI0C00319 g)-XDH (YALI0E12463 g), lacI q This work
 15 AmpR, ColE1 ori; PlacUV5: XYR13 (YALI0A19910 g)-XDH (YALI0E12463 g), lacI q This work
 16 AmpR, ColE1 ori; PlacUV5: SDR (YALI0D18964 g)-XDH (YALI0E12463 g), lacI q This work
 17 AmpR, ColE1 ori; PlacUV5: XYR (XP_001385181)-XDH (YALI0E12463 g), lacI q This work
 18 AmpR, ColE1 ori, LEU2, CEN; UAS1B8-TEF-(empty)-CYC1 [21]
 19 AmpR, ColE1 ori, LEU2, CEN; UAS1B8-TEF-hrGFP-CYC1 [21]
 20 AmpR, ColE1 ori, URA3, CEN; UAS1B8-TEF-hrGFP-CYC1 [21]
 21 AmpR, ColE1 ori, LEU2, CEN; UAS1B8-TEF-XKS (YALI0F10923 g)-CYC1 This work
 22 AmpR, ColE1 ori, URA3, CEN; UAS1B8-TEF-XDH (YALI0E12463 g)-CYC1 This work
 23 AmpR, ColE1 ori, LEU2, CEN; UAS1B8-TEF-cas9-CYC1, TEF-HH-sgRNA-HDV [19]
 24 AmpR, ColE1 ori, LEU2, CEN; UAS1B8-TEF-cas9-CYC1, TEF-HH-(XDH)sgRNA-HDV This work
 25 AmpR, ColE1 ori, LEU2, CEN; UAS1B8-TEF-cas9-CYC1, SCRp’-tRNAp-
(PEX10)sgRNA
[19]
 27 AmpR, ColE1 ori, LEU2, CEN; UAS1B8-TEF-cas9-CYC1, SCRp’-tRNAp-(XKS)sgRNA This work
 28 AmpR, ColE1ori; PT7lac: empty, lacI q (pETDuet) Novagen
 29 AmpR, ColE1ori; PT7lac: 6xhis-XDH (YALI0E12463 g), lacI q This work
 30 AmpR, ColE1ori; PT7lac: 6xhis-XYR1 (YALI0D07634 g), lacI q This work
 31 AmpR, ColE1ori; PT7lac: 6xhis-XYR2 (YALI0F18590 g), lacI q This work
 32 AmpR, ColE1 ori, LEU2, CEN; UAS1B8-TEF-XYR1-CYC1 This work
 33 AmpR, ColE1 ori, URA3, CEN; UAS1B8-TEF-XYR2-CYC1 This work