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Table 1 Strains and plasmids

From: Engineering xylose utilization in Yarrowia lipolytica by understanding its cryptic xylose pathway

 

Genotype

References

E. coli strains

 BW25113

rrnB T14 ΔlacZ WJ16 hsdR514 ΔaraBAD AH33 ΔrhaBAD LC78

[33, 39]

 BW25113

ΔxylA

BW25113 with xylA::kanR

[33, 39]

 BW25113

ΔxylB

BW25113 with xylB::kanR

[33, 39]

 BL21(DE3)

fhuA2 [lon] ompT gal (λ DE3) [dcm] ∆hsdS

λ DE3 = λ sBamHIo ∆EcoRI-B int::(lacI::PlacUV5::T7 gene1) i21 ∆nin5

[40]

Y. lipolytica strains

 P01f

MatA, leu2-270, ura3-302, xpr2-322, axp1-2 (ATCC no. MYA-2613)

ATCC

 P01f–XKS

P01f with alternate frameshift mutation in XKS gene

This work

 P01f–XDH

P01f with frameshift mutation in XDH gene

This work

Plasmids

 1

AmpR, ColE1 ori; PlacUV5: fadD-AtfA, lacI q

[4]

 2

AmpR, ColE1 ori; PlacUV5: XKS (YALI0F10923 g), lacI q

This work

 3

AmpR, ColE1 ori; PlacUV5: XYR1 (YALI0D07634 g)-XDH (YALI0E12463 g), lacI q

This work

 4

AmpR, ColE1 ori; PlacUV5: XYR2 (YALI0F18590 g)-XDH (YALI0E12463 g), lacI q

This work

 5

AmpR, ColE1 ori; PlacUV5: XYR3 (YALI0A15906 g)-XDH (YALI0E12463 g), lacI q

This work

 6

AmpR, ColE1 ori; PlacUV5: XYR4 (YALI0B21780 g)-XDH (YALI0E12463 g), lacI q

This work

 7

AmpR, ColE1 ori; PlacUV5: XYR5 (YALI0C13508 g)-XDH (YALI0E12463 g), lacI q

This work

 8

AmpR, ColE1 ori; PlacUV5: XYR6 (YALI0B07117 g)-XDH (YALI0E12463 g), lacI q

This work

 9

AmpR, ColE1 ori; PlacUV5: XYR7 (YALI0E18348 g)-XDH (YALI0E12463 g), lacI q

This work

 10

AmpR, ColE1 ori; PlacUV5: XYR8 (YALI0042092 g)-XDH (YALI0E12463 g), lacI q

This work

 11

AmpR, ColE1 ori; PlacUV5: XYR9 (YALI0C09119 g)-XDH (YALI0E12463 g), lacI q

This work

 12

AmpR, ColE1 ori; PlacUV5: XYR10 (YALI0F06974 g)-XDH (YALI0E12463 g), lacIq

This work

 13

AmpR, ColE1 ori; PlacUV5: XYR11 (YALI0B15268 g)-XDH (YALI0E12463 g), lacI q

This work

 14

AmpR, ColE1 ori; PlacUV5: XYR12 (YALI0C00319 g)-XDH (YALI0E12463 g), lacI q

This work

 15

AmpR, ColE1 ori; PlacUV5: XYR13 (YALI0A19910 g)-XDH (YALI0E12463 g), lacI q

This work

 16

AmpR, ColE1 ori; PlacUV5: SDR (YALI0D18964 g)-XDH (YALI0E12463 g), lacI q

This work

 17

AmpR, ColE1 ori; PlacUV5: XYR (XP_001385181)-XDH (YALI0E12463 g), lacI q

This work

 18

AmpR, ColE1 ori, LEU2, CEN; UAS1B8-TEF-(empty)-CYC1

[21]

 19

AmpR, ColE1 ori, LEU2, CEN; UAS1B8-TEF-hrGFP-CYC1

[21]

 20

AmpR, ColE1 ori, URA3, CEN; UAS1B8-TEF-hrGFP-CYC1

[21]

 21

AmpR, ColE1 ori, LEU2, CEN; UAS1B8-TEF-XKS (YALI0F10923 g)-CYC1

This work

 22

AmpR, ColE1 ori, URA3, CEN; UAS1B8-TEF-XDH (YALI0E12463 g)-CYC1

This work

 23

AmpR, ColE1 ori, LEU2, CEN; UAS1B8-TEF-cas9-CYC1, TEF-HH-sgRNA-HDV

[19]

 24

AmpR, ColE1 ori, LEU2, CEN; UAS1B8-TEF-cas9-CYC1, TEF-HH-(XDH)sgRNA-HDV

This work

 25

AmpR, ColE1 ori, LEU2, CEN; UAS1B8-TEF-cas9-CYC1, SCRp’-tRNAp-

(PEX10)sgRNA

[19]

 27

AmpR, ColE1 ori, LEU2, CEN; UAS1B8-TEF-cas9-CYC1, SCRp’-tRNAp-(XKS)sgRNA

This work

 28

AmpR, ColE1ori; PT7lac: empty, lacI q (pETDuet)

Novagen

 29

AmpR, ColE1ori; PT7lac: 6xhis-XDH (YALI0E12463 g), lacI q

This work

 30

AmpR, ColE1ori; PT7lac: 6xhis-XYR1 (YALI0D07634 g), lacI q

This work

 31

AmpR, ColE1ori; PT7lac: 6xhis-XYR2 (YALI0F18590 g), lacI q

This work

 32

AmpR, ColE1 ori, LEU2, CEN; UAS1B8-TEF-XYR1-CYC1

This work

 33

AmpR, ColE1 ori, URA3, CEN; UAS1B8-TEF-XYR2-CYC1

This work