Fig. 3
From: Cell-free synthesis of functional phospholipase A1 from Serratia sp.
Purification of PLA1 expressed in each system. Cell-free synthesized PLA1 from a 3 mL reaction was purified through a Ni-NTA column (a). Turbidity of the crude sesame oil was cleared after incubation with the purified PLA1 (30 µg PLA1 in 10 mL oil) for 12 h at 40 °C, indicating that the cell-free synthesized enzyme catalyzed degumming of crude oil (b)