Fig. 2

Functional validation of A. niger XltA as a xylose transporter, and of yeast strain EBY.XP as a xylose-utilizing strain. Serial dilutions of the EBY.XP xltA ⁺ strain, expressing the A. niger sugar porter gene cloned in expression vector p426HXT7-6His, were grown in minimal medium agar plates containing maltose or xylose 1 % (w/v). Agar plates were incubated at 30 °C for 5 days. EV: EBY.XP, carrying the empty expression vector p426HXT7-6His, was used as a negative control