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Table 2 Xylose and glucose initial uptake kinetics of fungal MFS transporters

From: Identification and functional characterization of novel xylose transporters from the cell factories Aspergillus niger and Trichoderma reesei

Transporter Xylose initial uptake kinetics Glucose initial uptake kinetics References
V max (nmol min−1 mg DW−1) K m (mM) V max (nmol min−1 mg DW−1) K m (mM)
AnXltA 1.08 ± 0.05 0.09 ± 0.03 1.11 ± 0.15 0.07 ± 0.01 This study
AnXltB 0.10 ± 0.00 15.0 ± 4.50 ND ND This study
AnXltC 0.14 ± 0.01 4.71 ± 1.04 1.18 ± 0.15 0.11 ± 0.02 This study
TrStr1 0.04 ± 0.01 5.70 ± 0.19 0.14 ± 0.04 0.01 ± 0.00 This study
TrStr2 0.12 ± 0.02 6.18 ± 0.81 0.69 ± 0.19 0.05 ± 0.01 This study
TrStr3 0.46 ± 0.04 2.19 ± 0.29 1.31 ± 0.33 0.06 ± 0.01 This study
AnMstA NCa 0.3 ± 0.1 NC 0.03 ± 0.01 [38]
GXS1 0.01 ± 0.00 0.08 ± 0.02 [17]
XUT3 0.01 ± 0.00 4.09 ± 1.08 [17]
Xyp29 0.61 ± 0.05 175.74 ± 21.36 ND ND [26]
An25 0.69 ± 0.04 55.96 ± 9.37 ND ND [26]
SUT1 132.0 ± 1.0 145.0 ± 1.0 45.0 ± 1.0 1.5 ± 0.1 [23]
SUT2+ 41.0 ± 1.0 49.0 ± 1.0 3.3 ± 0.1
28.0 ± 4.0
1.1 ± 0.1
55 ± 11.0
[23]
SUT3+ 87.0 ± 2.0 103.0 ± 3.0 3.7 ± 0.1
22.0 ± 0.1
0.8 ± 0.1
31.0 ± 0.1
[23]
Ag11C3 ND ND ND ND This study
  1. Ag11C3; laboratory-evolved xylose-utilizing strain, transformed with the empty vector p426HXT7-6His
  2. Italics: originating from filamentous fungi
  3. + Two glucose transport components were reported
  4. − Not determined
  5. ND not detectable
  6. NC not comparable
  7. aValues were reported in an incompatible unit (nmol min−1 mg protein−1)