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Table 2 Xylose and glucose initial uptake kinetics of fungal MFS transporters

From: Identification and functional characterization of novel xylose transporters from the cell factories Aspergillus niger and Trichoderma reesei

Transporter

Xylose initial uptake kinetics

Glucose initial uptake kinetics

References

V max (nmol min−1 mg DW−1)

K m (mM)

V max (nmol min−1 mg DW−1)

K m (mM)

AnXltA

1.08 ± 0.05

0.09 ± 0.03

1.11 ± 0.15

0.07 ± 0.01

This study

AnXltB

0.10 ± 0.00

15.0 ± 4.50

ND

ND

This study

AnXltC

0.14 ± 0.01

4.71 ± 1.04

1.18 ± 0.15

0.11 ± 0.02

This study

TrStr1

0.04 ± 0.01

5.70 ± 0.19

0.14 ± 0.04

0.01 ± 0.00

This study

TrStr2

0.12 ± 0.02

6.18 ± 0.81

0.69 ± 0.19

0.05 ± 0.01

This study

TrStr3

0.46 ± 0.04

2.19 ± 0.29

1.31 ± 0.33

0.06 ± 0.01

This study

AnMstA

NCa

0.3 ± 0.1

NC

0.03 ± 0.01

[38]

GXS1

0.01 ± 0.00

0.08 ± 0.02

–

–

[17]

XUT3

0.01 ± 0.00

4.09 ± 1.08

–

–

[17]

Xyp29

0.61 ± 0.05

175.74 ± 21.36

ND

ND

[26]

An25

0.69 ± 0.04

55.96 ± 9.37

ND

ND

[26]

SUT1

132.0 ± 1.0

145.0 ± 1.0

45.0 ± 1.0

1.5 ± 0.1

[23]

SUT2+

41.0 ± 1.0

49.0 ± 1.0

3.3 ± 0.1

28.0 ± 4.0

1.1 ± 0.1

55 ± 11.0

[23]

SUT3+

87.0 ± 2.0

103.0 ± 3.0

3.7 ± 0.1

22.0 ± 0.1

0.8 ± 0.1

31.0 ± 0.1

[23]

Ag11C3

ND

ND

ND

ND

This study

  1. Ag11C3; laboratory-evolved xylose-utilizing strain, transformed with the empty vector p426HXT7-6His
  2. Italics: originating from filamentous fungi
  3. + Two glucose transport components were reported
  4. − Not determined
  5. ND not detectable
  6. NC not comparable
  7. aValues were reported in an incompatible unit (nmol min−1 mg protein−1)