Fig. 4

Detection by Western blot analysis of cellulolytic enzymes accumulated in transplastomic DC plants. Each transplastomic line shows a prominent additional protein band corresponding in size to the foreign protein. Endoglucanase (a) and endo-β-1,4-xylanase (b) enzymes accumulation in DC1, DC2, DC3 and DC11 transplastomic plants, respectively (T1 generation). c Accumulation of β-glucosidase enzyme in DC21 and DC23 transplastomic plants (T0 generation). FLAG: recombinant Flag standard protein; PRV: transplastomic control plant transformed with the empty vector