Exploring xylose metabolism in Spathaspora species: XYL1.2 from Spathaspora passalidarum as the key for efficient anaerobic xylose fermentation in metabolic engineered Saccharomyces cerevisiae

Cadete, Raquel M.; de las Heras, Alejandro M.; Sandström, Anders G.; Ferreira, Carla; Gírio, Francisco; Gorwa-Grauslund, Marie-Françoise; Rosa, Carlos A.; Fonseca, César

doi:10.1186/s13068-016-0570-6

Biotechnology for Biofuels and Bioproducts

Table 1 d-Xylose consumption and product formation (biomass, ethanol, and xylitol) (g L⁻¹) in YPX fermentation assays (d-xylose, 40–50 g L⁻¹) with Spathaspora species under moderate and severe oxygen-limited conditions, respectively

From: Exploring xylose metabolism in Spathaspora species: XYL1.2 from Spathaspora passalidarum as the key for efficient anaerobic xylose fermentation in metabolic engineered Saccharomyces cerevisiae

Oxygen-limited conditions	Yeast species	Strain number	Time of maximum ethanol production (h)	d-xylose consumption (%)	r _s (g g ⁻¹_CDW h⁻¹)	Biomass (g_CDW L⁻¹)	Y _x/s (g g⁻¹)	Ethanol (g L⁻¹)	\(Y_{{{\text{p}}/{\text{s}}}}^{\text{et}}\) (g g⁻¹)	r _et (g g ⁻¹_CDW h⁻¹)	Xylitol (g L⁻¹)	Y ^xy_p/s (g g⁻¹)
Moderate	Spathaspora arborariae	UFMG-CM-Y352^T	72	99.2	0.28	12.1 ± 0.6	0.24	10.6 ± 0.1	0.21	0.12	6.4 ± 0.1	0.13
	Sp. brasiliensis	UFMG-CM-Y353^T	60	91.5	0.29	8.3 ± 0.6	0.18	3.7 ± 0.5	0.08	0.03	22.9 ± 0.2	0.46
	Sp. passalidarum	UFMG-CM-Y469	18	99.3	0.45	8.3 ± 0.7	0.19	20.2 ± 0.1	0.47	0.22	1.1 ± 0.0	0.03
	Sp. roraimanensis	UFMG-CM-Y477^T	48	100	0.43	4.8 ± 0.1	0.09	5.8 ± 0.3	0.11	0.07	22.7 ± 0.3	0.43
	Sp. suhii	UFMG-CM-Y475^T	48	100	0.44	4.7 ± 0.2	0.10	5.9 ± 0.1	0.12	0.09	22.4 ± 0.2	0.46
	Sp. xylofermentans	UFMG-CM-Y478^T	48	55.4	0.18	8.3 ± 0.8	0.31	2.6 ± 0.2	0.10	0.02	9.0 ± 0.8	0.33
	Sp. passalidarum	CBS 10155^T	18	99.1	0.43	8.9 ± 0.2	0.21	20.3 ± 0.2	0.48	0.21	0.6 ± 0.0	0.02
Severe	Sp. arborariae	UFMG-CM-Y352^T	120	99.3	0.32	5.5 ± 0.0	0.11	16.0 ± 0.72	0.32	0.11	9.0 ± 0.03	0.18
	Sp. brasiliensis	UFMG-CM-Y353^T	72	31.5	0.19	3.2 ± 0.6	0.21	2.6 ± 0.82	0.16	0.09	11.9 ± 0.88	0.47
	Sp. passalidarum	UFMG-CM-Y469	24	99.4	0.69	4.1 ± 0.1	0.10	20.5 ± 0.6	0.48	0.30	1.4 ± 0.01	0.03
	Sp. roraimanensis	UFMG-CM-Y477^T	144	100	0.39	5.1 ± 0.6	0.11	3.0 ± 0.1	0.06	0.02	27.4 ± 0.4	0.56
	Sp. suhii	UFMG-CM-Y475^T	–	14.8	0.16	0.3 ± 0.1	0.04	–	–	–	6.4 ± 0.6	0.92
	Sp. xylofermentans	UFMG-CM-Y478^T	144	97.5	0.18	6.9 ± 0.7	0.15	3.7 ± 0.4	0.08	0.02	24.4 ± 1.3	0.51
	Sp. passalidarum	CBS 10155^T	24	99.4	0.57	4.5 ± 0.2	0.11	20.5 ± 0.1	0.48	0.28	1.0 ± 0.2	0.02

r _s specific xylose consumption rate, r _et specific ethanol production rate, Y _x/s biomass yield; \(Y_{p/s}^{et}\) ethanol yield, \(Y_{p/s}^{xy}\) xylitol yield

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