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Table 2 Xylose reductase (XR) and xylitol dehydrogenase (XDH) activities in Spathaspora species, expressed in units (U) per mg protein [U (mg protein)−1], after 16 h of fermentation in YPX medium under moderate and severe oxygen-limited conditions, respectively

From: Exploring xylose metabolism in Spathaspora species: XYL1.2 from Spathaspora passalidarum as the key for efficient anaerobic xylose fermentation in metabolic engineered Saccharomyces cerevisiae

Oxygen-limited conditions Yeast species Strain number XR XDH
NADH NADPH Ratio NADH/NADPH NAD+
Moderate Spathaspora arborariae UFMG-CM-Y352T 0.54 ± 0.02 0.72 ± 0.06 0.75 ± 0.07 0.57 ± 0.05
Sp. brasiliensis UFMG-CM-Y353T 0.39 ± 0.02 0.18 ± 0.01
Sp. passalidarum UFMG-CM-Y469 0.83 ± 0.12 0.46 ± 0.06 1.81 ± 0.35 0.60 ± 0.07
Sp. roraimanensis UFMG-CM-Y477T 0.64 ± 0.04 0.74 ± 0.02
Sp. suhii UFMG-CM-Y475T 0.51 ± 0.03 0.74 ± 0.04
Sp. xylofermentans UFMG-CM-Y478T 0.09 ± 0.01 0.12 ± 0.01
Sp. passalidarum CBS 10155T 2.81 ± 0.31 1.62 ± 0.17 1.74 ± 0.26 1.80 ± 0.14
Severe Sp. arborariae UFMG-CM-Y352T 0.33 ± 0.04 0.61 ± 0.01 0.55 ± 0.07 0.84 ± 0.07
Sp. brasiliensis UFMG-CM-Y353T 0.35 ± 0.01 0.60 ± 0.01
Sp. passalidarum UFMG-CM-Y469 1.09 ± 0.12 0.63 ± 0.14 1.72 ± 0.43 1.17 ± 0.12
Sp. roraimanensis UFMG-CM-Y477T 0.26 ± 0.03 0.87 ± 0.01
Sp. suhii UFMG-CM-Y475T 0.20 ± 0.01 0.63 ± 0.04
Sp. xylofermentans UFMG-CM-Y478T 0.27 ± 0.01 0.54 ± 0.01
Sp. passalidarum CBS 10155T 4.07 ± 0.55 2.46 ± 0.27 1.66 ± 0.29 2.19 ± 0.05