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Table 4 D-xylose consumption, product formation (biomass, ethanol, and xylitol) and xylose reductase (XR) activity in anaerobic xylose fermentation (50 g L−1) with recombinant S. cerevisiae strains TMB 3422 and TMB 3504

From: Exploring xylose metabolism in Spathaspora species: XYL1.2 from Spathaspora passalidarum as the key for efficient anaerobic xylose fermentation in metabolic engineered Saccharomyces cerevisiae

Strains

µ max

(h−1)

r s (g g −1CDW  h−1)

Y x/s (g g−1)

\(Y_{\text{p/s}}^{\text{et}}\) (g g−1)

r et

(g gCDW −1 h−1)

\(Y_{\text{p/s}}^{\text{xy}}\) (g g−1)

XR

NADH

NADPH

Ratio NADH/NADPH

TMB 3422

0.07 ± 0.00

0.58 ± 0.10

0.04 ± 0.01

0.34 ± 0.01

0.18 ± 0.01

0.21 ± 0.01

0.67 ± 0.03

0.94 ± 0.02

0.71 ± 0.04

TMB 3504

0.11 ± 0.00

0.76 ± 0.06

0.06 ± 0.00

0.40 ± 0.00

0.33 ± 0.02

0.10 ± 0.02

1.13 ± 0.08

0.96 ± 0.01

1.18 ± 0.08

  1. µ max specific growth rate, r s specific xylose consumption rate, r et specific ethanol production rate, Y x/s biomass yield; \(Y_{p/s}^{et}\) ethanol yield; \(Y_{p/s}^{xy}\) xylitol yield