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Table 5 Yeast strains and plasmids constructed and/or used in this study

From: Exploring xylose metabolism in Spathaspora species: XYL1.2 from Spathaspora passalidarum as the key for efficient anaerobic xylose fermentation in metabolic engineered Saccharomyces cerevisiae

Strains and plasmids

Relevant features

References

Plasmids

 YpOB8

URA3 TDH3p-XYL1-ADH1t, PGK1p-XYL2-PGK1t

[41]

 YpDR7

URA3 TDH3p-XYL1(N272D)-ADH1t, PGK1p-XYL2-PGK1t

[14]

 YIpRC1

pOB8 XYL1.1 S. passalidarum CBS 10155T

This work

 YIpRC2

pOB8 XYL1.1 S. passalidarum UFMG-CM-Y469

This work

 YIpRC4

pOB8 XYL1.2 S. passalidarum CBS 10155T

This work

 YIpRC5

pOB8 XYL1.2 S. passalidarum UFMG-CM-Y469

This work

S. cerevisiae strains

 TMB 3044

CEN.PK 2-1C, MATa, ura3-52, Δgre3, his3::HIS3 PGK1p-XKS1-PGK1t, TAL1::PGK1p-TAL1-PGK1t, TKL1::PGK1p-TKL1-PGK1t, RKI1::PGK1p-RKI1-PGK1t, RPE1::PGK1p-RPE1-PGK1t

[7]

 TMB 3422

TMB 3044, ura3::YIpDR7

[14]

 TMB 3501

TMB 3044, ura3:: YIpRC1

This work

 TMB 3502

TMB 3044, ura3:: YIpRC2

This work

 TMB 3503

TMB 3044, ura3:: YIpRC4

This work

 TMB 3504

TMB 3044, ura3:: YIpRC5

This work