Fig. 3

Electrophoretic mobility of components and assembled complexes as assessed by non-denaturing gels. Equimolar concentrations of the chimeric enzymes and their matching scaffoldin were combined to form a conventional designer cellulosomes or b thermostable designer cellulosomes. Near-complete interaction is indicated by the formation of a shifted major band. Wild-type forms of the C. thermocellum modules (Cel48S, Cel8A and Bgl1A catalytic modules, cohesin and CBM of the chimaeric scaffoldin) are shown in pink pictograms. Thermostabilized mutated forms of the respective catalytic module are shown in red pictograms and denoted by an asterisk. The pink, black and blue asterisks indicate the inherently thermostable C. thermocellum cohesin and CBM, A. fulgidus and C. clariflavum cohesins, respectively