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Table 1 Primers used in the study (restriction sites represented in upper case)

From: Enhancement of cellulosome-mediated deconstruction of cellulose by improving enzyme thermostability

Construct name (intermediate construct) Vector Forward primer (5’–3’) Reverse Primer (5’–3’) Linearized vector Primary PCR templatea Secondary PCR templatea
BglA-v (BglA) pET21a CTTTAAGAAGGAGATATACATATGGCTAGCcaccatcaccatcaccacaagataactttcccaaaaga ccatgtaggaacgagctttgtgccACTAGTCTGAGGAGTTGTTACAGTTGTgaaaccgttgtttttgattacttc   pET28a BglA [32] pET21a
BglA-v pET21a ttacatACTAGTtctcataaatttatctatgg ttactaGAGCTCctattgttcttcaactggga pET9d
Xyn-docB [48]
 
BglA*-v (BglA*) pET21a CTTTAAGAAGGAGATATACATATGGCTAGCcaccatcaccatcaccacaagataactttcccaaaaga ccatgtaggaacgagctttgtgccACTAGTCTGAGGAGTTGTTACAGTTGTgaaaccgttgtttttgattacttc   pET28a
BglA*
pET21a
BglA*-f pET21a CTTTAAGAAGGAGATATACATATGGCTAGCcaccatcaccatcaccacaagataactttcccaaaaga ccatgtaggaacgagctttgtgccACTAGTCTGAGGAGTTGTTACAGTTGTgaaaccgttgtttttgattacttc   pET28a
BglA*
pET21a
Xyn10A-f [42]
BglA*-v pET21a ttacatACTAGTtctcataaatttatctatgg ttactaGAGCTCctattgttcttcaactggga pET9d
Xyn-docB [48]
 
8A-g (8A) pET28a ttacgtCCATGGgtgtgccttttaacacaaaata ttacgaGGTACCaatgaaggtgtcggattcga pET28a
Cel8A [29]
 
8A-g pET28a tactagGGTACCagaagaagcaaacaagggag ttactaCTCGAGcttacccagtaagccattct pET28a-g-5A [6]  
8A*-g (8A*) pET28a ttacgtCCATGGgtgtgccttttaacacaaaata ttacgaGGTACCaatgaaggtgtcggattcga pET28a
8AQM [24]
 
8A*-g pET28a tactagGGTACCagaagaagcaaacaagggag ttactaCTCGAGcttacccagtaagccattct pET28a-g-5A [6]  
8A*-b pET28a ttacgtCCATGGgtgtgccttttaacacaaaata ttacgaGGTACCaatgaaggtgtcggattcga pET28a
8A-b [29]
  
Scaf·GT pET9d GTTTAACTTTAAGAAGGAGATATAccatggtgcttcctccgaaaactacc ggtgtgtttgtcggtgtgtttgtcGGTACCgcttcttcctgagagacaatc   pET28a
CBM-Coh2375 [34]
pET28a
Scaf·AT [49]
Scaf·GTV pET9d gttctttgacggtggagtaaatgttggagatacaacagtacctacaacacctacaacacctGCAGGGCAATTACAAATTGA CCTTTCGGGCTTTGTTAGCAGCCggatcctAATTTGAGCCAACCAATATAG   pET28a CBM-CohC1
[48]
pET9d
Scaf·GT
  1. aIndicates restriction-free cloning