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Table 3 Conservation of N-glycosylated sites of selected A. nidulans CAZymes in homologous proteins

From: Mapping N-linked glycosylation of carbohydrate-active enzymes in the secretome of Aspergillus nidulans grown on lignocellulose

Identified proteins Accession number Domain N-glyc sitesa Conservation (%)b Homologous in Aspergilli c Sequond
Endo-1,3-β-glucanase Engl1 ANID_00472 GH81 219 90.0 20 NSS
    240 95.0 20 NAT
α-Glucosidase AgdA ANID_02017 GH31 432 91.3 22 NAS
β-Xylosidase ANID_02359 GH3 231 43.3 30 NHS
    673 55.9 34 NFT
    695 52.9 34 NTT
β-Glucosidase ANID_02828 GH3 225 100 50 NGT
    365 34.0 50 NGS
β-Glucosidase ANID_04102 GH3 62 98.0 50 NLT
    491 8.0 50 NKT
    642 92.0 50 NQT
    713 40.0 50 NST
1,4-β-d-Glucan-cellobiohydrolyase ANID_05176 GH7 284 62.0 50 NTS
Cellobiose dehydrogenase ANID_07230 AA8 132 37.5 24 NAT
    299 47.4 38 NGT
    308 90.0 40 NGT
    620 7.9 38 NVT
    709 23.7 38 NVS
β-Glucosidase ANID_07396 GH3 259 100 50 NNS
    438 72.0 50 NGT
    586 56.0 50 NSS
Endo-1,4-β-xylanase ANID_07401 GH10 123 100 11 NTT
β-Xylosidase ANID_08401 GH3 63 70.0 30 NNT
    340 30.0 30 NET
    408 46.7 30 NGT
    419 93.3 30 NFT
Arabinofuranosidase ANID_08477 GH43 438 13.6 23 NGS
Exo-polygalacturonase ANID_08761 GH28 113 62.1 29 NDT
    199 72.4 29 NSS
    292 100 30 NIS
    297 63.3 30 NAS
Exopolygalacturonase ANID_08891 GH28 65 21.9 32 NDT
    230 94.1 17 NAS
α-Glucuronidase ANID_09286 GH67 48 85.0 20 NAT
    315 95.0 20 NRT
    689 90.0 20 NKS
    769 15.0 20 NST
Exo-rhamnogalacturonase B ANID_10274 GH28 34 53.3 15 NET
    340 100 16 NCT
β-Glucosidase ANID_10482 GH3 73 100 50 NLT
    726 60.0 50 NSS
  1. aN-glycosylated sites confirmed in the A. nidulans proteins by LC–MS/MS
  2. bAlignment gaps on the sequences shift the N-glycosylation sequons in homologous sequences. Despite the sequons are in different positions on primary sequence, the N-glycan is attached to similar positions at proteins 3D-level (see Fig. 7)
  3. cProteins were considered homologous when the E value (Blastp) was equal to or less than 1.00E−70
  4. dSequon detected in A. nidulans glycoproteins by LC–MS/MS