Mapping N-linked glycosylation of carbohydrate-active enzymes in the secretome of Aspergillus nidulans grown on lignocellulose

Table 3 Conservation of N-glycosylated sites of selected A. nidulans CAZymes in homologous proteins

Identified proteins	Accession number	Domain	N-glyc sites^a	Conservation (%)^b	Homologous in Aspergilli ^c	Sequon^d
Endo-1,3-β-glucanase Engl1	ANID_00472	GH81	219	90.0	20	NSS
			240	95.0	20	NAT
α-Glucosidase AgdA	ANID_02017	GH31	432	91.3	22	NAS
β-Xylosidase	ANID_02359	GH3	231	43.3	30	NHS
			673	55.9	34	NFT
			695	52.9	34	NTT
β-Glucosidase	ANID_02828	GH3	225	100	50	NGT
			365	34.0	50	NGS
β-Glucosidase	ANID_04102	GH3	62	98.0	50	NLT
			491	8.0	50	NKT
			642	92.0	50	NQT
			713	40.0	50	NST
1,4-β-d-Glucan-cellobiohydrolyase	ANID_05176	GH7	284	62.0	50	NTS
Cellobiose dehydrogenase	ANID_07230	AA8	132	37.5	24	NAT
			299	47.4	38	NGT
			308	90.0	40	NGT
			620	7.9	38	NVT
			709	23.7	38	NVS
β-Glucosidase	ANID_07396	GH3	259	100	50	NNS
			438	72.0	50	NGT
			586	56.0	50	NSS
Endo-1,4-β-xylanase	ANID_07401	GH10	123	100	11	NTT
β-Xylosidase	ANID_08401	GH3	63	70.0	30	NNT
			340	30.0	30	NET
			408	46.7	30	NGT
			419	93.3	30	NFT
Arabinofuranosidase	ANID_08477	GH43	438	13.6	23	NGS
Exo-polygalacturonase	ANID_08761	GH28	113	62.1	29	NDT
			199	72.4	29	NSS
			292	100	30	NIS
			297	63.3	30	NAS
Exopolygalacturonase	ANID_08891	GH28	65	21.9	32	NDT
			230	94.1	17	NAS
α-Glucuronidase	ANID_09286	GH67	48	85.0	20	NAT
			315	95.0	20	NRT
			689	90.0	20	NKS
			769	15.0	20	NST
Exo-rhamnogalacturonase B	ANID_10274	GH28	34	53.3	15	NET
			340	100	16	NCT
β-Glucosidase	ANID_10482	GH3	73	100	50	NLT
			726	60.0	50	NSS

^aN-glycosylated sites confirmed in the A. nidulans proteins by LC–MS/MS
^bAlignment gaps on the sequences shift the N-glycosylation sequons in homologous sequences. Despite the sequons are in different positions on primary sequence, the N-glycan is attached to similar positions at proteins 3D-level (see Fig. 7)
^cProteins were considered homologous when the E value (Blastp) was equal to or less than 1.00E−70
^dSequon detected in A. nidulans glycoproteins by LC–MS/MS

ISSN: 2731-3654