A new laboratory evolution approach to select for constitutive acetic acid tolerance in Saccharomyces cerevisiae and identification of causal mutations

Table 1 Acetic acid tolerant mutants derived from CEN.PK113-7D through evolution/mutagenesis and crossing

Strain	Collection name	Relevant description	Source
CEN.PK113-7D		MATa	[45]
IMK439		MATα ura3∆::kanMX	[38]
MUT1A	IMS0379	Acetic acid tolerant mutant evolved from CEN.PK113-7D	This study
MUT2B	IMS0527	Acetic acid tolerant mutant evolved from CEN.PK113-7D	This study
MUT3E	IMS0528	Acetic acid tolerant mutant evolved from CEN.PK113-7D	This study
HAT1E	IMS0529	Acetic acid tolerant mutant evolved from CEN.PK113-7D	This study
HAT2A	IMS0530	Acetic acid tolerant mutant evolved from CEN.PK113-7D	This study
UV-E3	IMS0378	Acetic acid tolerant UV-radiated mutant from CEN.PK113-7D	This study
MUT1A-D	IMS0531	Diploid obtained from crossing MUT1A with IMK439	This study
MUT2B-D	IMS0532	Diploid obtained from crossing MUT2B with IMK439	This study
MUT3E-D	IMS0391	Diploid obtained from crossing MUT3E with IMK439	This study
HAT1E-D	IMS0392	Diploid obtained from crossing HAT1E with IMK439	This study
HAT2A-D	IMS0402	Diploid obtained from crossing HAT2A with IMK439	This study

IMK439 was used for mating experiments, which is congenic with CEN.PK113-7D, but has an opposite mating type and contains a URA3 deletion as well as the kanMX marker. The haploid segregants obtained by sporulation of the diploid strains are shown in Additional file 9

ISSN: 2731-3654