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Table 1 Acetic acid tolerant mutants derived from CEN.PK113-7D through evolution/mutagenesis and crossing

From: A new laboratory evolution approach to select for constitutive acetic acid tolerance in Saccharomyces cerevisiae and identification of causal mutations

Strain Collection name Relevant description Source
CEN.PK113-7D   MATa [45]
IMK439   MATα ura3∆::kanMX [38]
MUT1A IMS0379 Acetic acid tolerant mutant evolved from CEN.PK113-7D This study
MUT2B IMS0527 Acetic acid tolerant mutant evolved from CEN.PK113-7D This study
MUT3E IMS0528 Acetic acid tolerant mutant evolved from CEN.PK113-7D This study
HAT1E IMS0529 Acetic acid tolerant mutant evolved from CEN.PK113-7D This study
HAT2A IMS0530 Acetic acid tolerant mutant evolved from CEN.PK113-7D This study
UV-E3 IMS0378 Acetic acid tolerant UV-radiated mutant from CEN.PK113-7D This study
MUT1A-D IMS0531 Diploid obtained from crossing MUT1A with IMK439 This study
MUT2B-D IMS0532 Diploid obtained from crossing MUT2B with IMK439 This study
MUT3E-D IMS0391 Diploid obtained from crossing MUT3E with IMK439 This study
HAT1E-D IMS0392 Diploid obtained from crossing HAT1E with IMK439 This study
HAT2A-D IMS0402 Diploid obtained from crossing HAT2A with IMK439 This study
  1. IMK439 was used for mating experiments, which is congenic with CEN.PK113-7D, but has an opposite mating type and contains a URA3 deletion as well as the kanMX marker. The haploid segregants obtained by sporulation of the diploid strains are shown in Additional file 9