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Table 3 Mutations present in multiple evolved S. cerevisiae strains and genetic analysis of their relevance for acetic acid tolerance

From: A new laboratory evolution approach to select for constitutive acetic acid tolerance in Saccharomyces cerevisiae and identification of causal mutations

Gene Strain SNP Nucleotide in THS Nucleotide in SHS
ASG1 MUT1A G1248A A G
MUT2B G1248T T G
HAT1E A1979G G A
HAT2A G2881C C G
ADH3 MUT1A G416T T G
MUT2B T966G T-G T
HAT2A T201A A T
SKS1 MUT2B G821T T G
HAT2A C617A C-A nd
GIS4 MUT3E G1322C C G
UV-E3 G295A nd nd
SAC6 MUT1A G491A A G
MUT2B G491A nd A
MUT3E G491A G-A G
HAT1E G491A G A
HAT2A G491A G-A A
EUG1 MUT1A G-113T G G-T
MUT2B G-113T G-T T
MUT3E G-113T G T
HAT1E G-113T G-T G
HAT2A G-113T T T
  1. Mutations were identified by comparing whole-genome sequencing data of single-cell isolates MUT1A, MUT2B, MUT3E, HAT1E, HAT2A and UV-E3 to the genome sequence of S. cerevisiae CEN.PK113-7D [44]. Mutations shown occur in at least two strains. In addition to the nucleotide changes, the distribution of the SNP among tolerant haploid segregants (THS) and the sensitive haploid segregants (SHS) of a diploid, generated by mating the tolerant strains with S. cerevisiae IMK439, are shown. Nucleotides present in the evolved strains are bold and underlined. Two nucleotides separated by a dash indicate presence of both mutated and reference-strain alleles in pooled THS or SHS. nd no sequence data obtained