Characterization of a glucose-tolerant β-glucosidase from Anoxybacillus sp. DT3-1

Table 1 Characteristics of DT-Bgl compared with BGL from other organisms

Source	MM (kDa)	T_opt (°C)	pH_opt	Thermostability	K ^a_m (mM)	V _max (U^b/mg)	Reference
Anoxybacillus sp. DT3-1	53	70	8.5	60 °C for 24 h^c	0.22	923.7	This study
Thermoanaerobacterium aotearoense P8G3#4	46	60	6.0	50 °C	0.66	180.6	[29]
Thermoanaerobacterium thermosaccharolyticum DSM 571	52	70	6.4	60 °C	0.62	64	[12]
Dictyoglomus thermophilum DSM 3960	52	90	7.0	90 °C for 5 h^c	1.15	–	[30]
Thermotoga thermarum DSM 5069T	55	90	4.8	90 °C	0.59	142	[26]
Nasutitermes takasagoensis	60	65	5.5	60 °C	0.67	8	[31]
Rhizomucor miehei NRRL 5282	82	70	5.0	70 °C for 35 min^c	0.12	468.2	[32]

^a Determined by measuring the rate of hydrolysis using pNPG as substrate
^b One unit of enzyme activity is defined as the amount of enzyme necessary to liberate 1 μmol of p-nitrophenol per min under the assay conditions
^c Half-life activity of the enzyme

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