Table 3 Hydrolysis of various substrates by DT-Bgl
From: Characterization of a glucose-tolerant β-glucosidase from Anoxybacillus sp. DT3-1
Substrate (concentration, 1 % (w/v) | Linkage of the glycosyl group | Substrate depletiona (%) | Product formationb (µg) | ||
|---|---|---|---|---|---|
Cellobiose | Glucose | Galactose | |||
Cellobiose | β-(1 → 4)Glc | 93.61 ± 1.02 | – | 85.65 ± 0.96 | – |
Sophorose | β-(1 → 2)Glc | 99.93 ± 0.01 | – | 135.93 ± 0.29 | – |
Laminaribiose | β-(1 → 3)Glc | 100.00 ± 0.00 | – | 85.20 ± 0.71 | – |
Lactose | β-(1 → 4)Gal | 33.01 ± 5.79 | – | 37.01 ± 0.04 | 37.63 ± 0.03 |
β-gentiobiose | β-(1 → 6)Glc | 21.23 ± 1.41 | – | 37.84 ± 0.02 | – |
Salicin | β-salicyl alcohol glucoside | 93.81 ± 0.35 | – | 42.51 ± 0.07 | – |
Sucrose | α-(1 → 2)Fru | 0.40 ± 6.66 | – | 0.00 ± 0.00 | – |
Maltose | α-(1 → 4)Glc | 0.00 ± 0.00 | – | 0.00 ± 0.00 | – |
Cellotriose | β-(1 → 4)Glc | 100.00 ± 0.00 | 13.86 ± 0.00 | 76.11 ± 0.00 | – |
Cellotetraose | β-(1 → 4)Glc | 100.00 ± 0.00 | 11.82 ± 0.00 | 70.40 ± 0.00 | – |
Cellopentaose | β-(1 → 4)Glc | 100.00 ± 0.00 | 14.82 ± 0.00 | 114.56 ± 0.00 | – |
Cellohexaose | β-(1 → 4)Glc | 100.00 ± 0.00 | 11.63 ± 0.00 | 57.94 ± 0.02 | – |
Xylan | β-(1 → 4)Xyl | 0.00 ± 0.00 | 0.00 ± 0.00 | 0.00 ± 0.00 | – |
CMC-Na | β-(1 → 4)Glc | 0.00 ± 0.00 | 0.00 ± 0.00 | 0.00 ± 0.00 | – |
Avicel® PH-101 | β-(1 → 4)Glc | 0.00 ± 0.00 | 0.00 ± 0.00 | 0.00 ± 0.00 | – |
Sigmacell 101 | β-(1 → 4)Glc | 0.00 ± 0.00 | 0.00 ± 0.00 | 0.00 ± 0.00 | – |
α-Cellulose | β-(1 → 4)Glc | 0.00 ± 0.00 | 0.00 ± 0.00 | 0.00 ± 0.00 | – |