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Table 3 Hydrolysis of various substrates by DT-Bgl

From: Characterization of a glucose-tolerant β-glucosidase from Anoxybacillus sp. DT3-1

Substrate (concentration, 1 % (w/v) Linkage of the glycosyl group Substrate depletiona (%) Product formationb (µg)
Cellobiose Glucose Galactose
Cellobiose β-(1 → 4)Glc 93.61 ± 1.02 85.65 ± 0.96
Sophorose β-(1 → 2)Glc 99.93 ± 0.01 135.93 ± 0.29
Laminaribiose β-(1 → 3)Glc 100.00 ± 0.00 85.20 ± 0.71
Lactose β-(1 → 4)Gal 33.01 ± 5.79 37.01 ± 0.04 37.63 ± 0.03
β-gentiobiose β-(1 → 6)Glc 21.23 ± 1.41 37.84 ± 0.02
Salicin β-salicyl alcohol glucoside 93.81 ± 0.35 42.51 ± 0.07
Sucrose α-(1 → 2)Fru 0.40 ± 6.66 0.00 ± 0.00
Maltose α-(1 → 4)Glc 0.00 ± 0.00 0.00 ± 0.00
Cellotriose β-(1 → 4)Glc 100.00 ± 0.00 13.86 ± 0.00 76.11 ± 0.00
Cellotetraose β-(1 → 4)Glc 100.00 ± 0.00 11.82 ± 0.00 70.40 ± 0.00
Cellopentaose β-(1 → 4)Glc 100.00 ± 0.00 14.82 ± 0.00 114.56 ± 0.00
Cellohexaose β-(1 → 4)Glc 100.00 ± 0.00 11.63 ± 0.00 57.94 ± 0.02
Xylan β-(1 → 4)Xyl 0.00 ± 0.00 0.00 ± 0.00 0.00 ± 0.00
CMC-Na β-(1 → 4)Glc 0.00 ± 0.00 0.00 ± 0.00 0.00 ± 0.00
Avicel® PH-101 β-(1 → 4)Glc 0.00 ± 0.00 0.00 ± 0.00 0.00 ± 0.00
Sigmacell 101 β-(1 → 4)Glc 0.00 ± 0.00 0.00 ± 0.00 0.00 ± 0.00
α-Cellulose β-(1 → 4)Glc 0.00 ± 0.00 0.00 ± 0.00 0.00 ± 0.00
  1. aCalculated using the following formula: \(\frac{{Initialamountofsubstrate{-}Amountofsubstrate}}{Initialamountofsubstrate} \times 100\)
  2. bThe experiment was carried out by reacting 100 μL of substrate with 10 μL of enzyme as described in the “Methods” section