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Table 2 Strains and plasmids used in this study

From: Heterologous expression of family 10 xylanases from Acidothermus cellulolyticus enhances the exoproteome of Caldicellulosiruptor bescii and growth on xylan substrates

Name Description Reference
E. coli
 JW520 DH5α containing pSKW010 (ApramycinR) This study
 JW521 DH5α containing pSKW011 (ApramycinR) This study
C. bescii
 JWCB18 ΔpyrFA ldh::ISCbe4 Δcbe1(ura /5-FOAR) [40]
 JWCB52 ΔpyrFA ldh::ISCbe4 Δcbe1 CIS1::P S-layer acel0614(E1) (ura /5-FOAR) [10]
 JWCB21 JWCB18 containing pDCW89 (ura + /5-FOAS) [43]
 JWCB73 JWCB52 containing pJGW07 (ura + /5-FOAS) This study
 JWCB74 JWCB52 containing pSKW10 (ura + /5-FOAS) This study
 JWCB75 JWCB52 containing pSKW11 (ura + /5-FOAS) This study
Plasmids
 pDCW89 E. coli/C. bescii shuttle vector containing the C. bescii pyrF gene (ApramycinR) [43]
 pJGW07 E. coli/C. bescii shuttle vector containing the C. thermocellum pyrF gene (ApramycinR) [41]
 pDCW173 Intermediate vector 1 (ApramycinR) [16]
 pDCW212 Intermediate vector 2 (ApramycinR) This study
 pDCW213 Source of Acel_0180 open reading frame (ApramycinR) This study
 pDCW214 Source of Acel_0372 open reading frame (ApramycinR) This study
 pSKW10 Expression vector containing P S-layer—Acel_0180 (ApramycinR) This study
 pSKW11 Expression vector containing P S-layer—Acel_0372 (ApramycinR) This study