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Fig. 6 | Biotechnology for Biofuels

Fig. 6

From: Functional characterization of the native swollenin from Trichoderma reesei: study of its possible role as C1 factor of enzymatic lignocellulose conversion

Fig. 6

AFM imaging of SWO1 action on ATFC. a A three-dimensional representation of the experimental setup. ATFC of defined height is placed on a silicon wafer, which can be used as reference. b The ATFC surface is homogenous and nanoflat with a mean surface roughness below 5 nm. c ATFC substrates on a single silicon wafer (~1 cm2) were incubated in 50 mM sodium acetate buffer, pH 5.0, at 40 °C with mild agitation in a total reaction volume of 2 mL. Two exemplary height profiles from ATFC substrates after incubation with (blue) or without 0.4 µM SWO1 (red) after 24 h are shown. No significant changes induced by SWO1 incubation were found. Note that the edges of amorphous cellulose films were slightly deformed due to a cutting process prior to the addition of SWO1. Thus, only the surface with a certain distance (~1.0 µm) to the edge was analyzed. d Height distribution profiles of spots on the ATFC surface after incubation with (blue) or without SWO1 (red) using the same experimental conditions as stated above. A broadening of the peak, which would indicate degradation or swelling, is not visible. Analyzed areas were at least 1 µm2

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