Fig. 9

Effect of SWO1 pretreatment on the enzymatic hydrolysis of cellulosic substrates. The substrates used were Avicel PH-101 (○/●) and CNCs (Δ/▼). The substrate concentration was 1 mg/mL. All reactions were done in 50 mM sodium acetate buffer, pH 5.0, at 40 °C with shaking (500 rpm) in a total reaction volume of 1.5 mL. Prior to the addition of cellulase, the substrate preparation was incubated with 0.4 µM SWO1 (●/▼) or BSA (○/Δ) for 24 h. T. reesei cellulase and β-glucosidase were then added in a small volume (60 µL) to a final enzyme loading of 20 µg/mg substrate and 4 µg/mg substrate, respectively. The mixture was incubated for another 24 h using the same conditions as stated above. The liberated glucose was measured with an enzymatic assay. Error bars show SD from four independent experiments