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Table 4 Strains used in this study

From: Elucidation of the co-metabolism of glycerol and glucose in Escherichia coli by genetic engineering, transcription profiling, and 13C metabolic flux analysis

Strains Relevant genotype or description Source or reference
BW25113 F λ rph 1 ΔaraBAD AH33 lacI q ΔlacZ WJ16 rrnB T14 ΔrhaBAD LD78 hsdR514 E. coli Genetic Stock Center from Yale University
Lin 43 Hfr(PO2A) fhuA22, ΔphoA8, fadL701(T2R), relA1, glpR2(glp c), pitA10, spoT1, glpK22(fbR), rrnB-2, mcrB1, creC510 E. coli Genetic Stock Center from Yale University
BW25113 ΔptsG BW25113, ptsG This study
BW25113 ΔptsGglpK* BW25113 ∆ptsG, glpK gene replaced by glpK22 from strain Lin43 This study