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Table 4 Strains used in this study

From: Elucidation of the co-metabolism of glycerol and glucose in Escherichia coli by genetic engineering, transcription profiling, and 13C metabolic flux analysis

Strains

Relevant genotype or description

Source or reference

BW25113

F − λ − rph −1 ΔaraBAD AH33 lacI q ΔlacZ WJ16 rrnB T14 ΔrhaBAD LD78 hsdR514

E. coli Genetic Stock Center from Yale University

Lin 43

Hfr(PO2A) fhuA22, ΔphoA8, fadL701(T2R), relA1, glpR2(glp c), pitA10, spoT1, glpK22(fbR), rrnB-2, mcrB1, creC510

E. coli Genetic Stock Center from Yale University

BW25113 ΔptsG

BW25113, ptsG −

This study

BW25113 ΔptsGglpK*

BW25113 ∆ptsG, glpK gene replaced by glpK22 from strain Lin43

This study

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Biotechnology for Biofuels and Bioproducts

ISSN: 2731-3654

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