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Table 2 The parameters of the processes conducted in flask and batch cultivation

From: A novel strain of Yarrowia lipolytica as a platform for value-added product synthesis from glycerol

 

A101

AJD pADUTGut1

AJD pADUTGut2

AJD pADUTGut1/2

Shake flaks experiment

 Glycerol Assimilation Medium (pH 6.0)

    

  Glolcons (g/L)a

72.57 ± 5.18

87.08 ± 3.32

84.64 ± 1.94

97.92 ± 0.67

 Erythritol Fermentation Medium (pH 3.0)

    

  Q ERY (g/L/h)

0.08 ± 0.01

0.32 ± 0.01

0.30 ± 0.04

0.30 ± 0.01

  Y ERY (g/g)

0.06 ± 0.01

0.23 ± 0.01

0.21 ± 0.03

0.22 ± 0.01

 CA Fermentation Medium (pH3.0)

    

  Q CA (g/L/h)

0.02 ± 0.01

0.15 ± 0.01

0.26 ± 0.01

0.28 ± 0.01

  Y CA (g/g)

0.02 ± 0.01

0.11 ± 0.01

0.18 ± 0.01

0.20 ± 0.01

Batch fermentation

 Glycerol Assimilation Medium (pH 6.0)

    

  Glolcons (g/L)b

142.43 ± 3.86

149.58 ± 0.50

150 ± 0.01

150 ± 0.01

 Erythritol Fermentation Medium (pH 3.0)

    

  Q ERY (g/L/h)

0.80 ± 0.03

0.99 ± 0.02

0.58 ± 0.01

1.08 ± 0.02

  Y ERY (g/g)

0.38 ± 0.01

0.48 ± 0.01

0.28 ± 0.02

0.52 ± 0.01

 CA Fermentation Medium (pH3.0)

    

  Q CA (g/L/h)

0.05 ± 0.01

0.63 ± 0.01

0.57 ± 0.01

0.69 ± 0.01

  Y CA (g/g)

0.03 ± 0.01

0.40 ± 0.01

0.36 ± 0.04

0.43 ± 0.01

  1. a Glol cons glycerol consumed at 54 hours of the process
  2. b Glol cons (g/L) glycerol consumed at 48 hours of the process
  3. Q ERY the erythritol productivity, Y ERY the erythritol yield, Q CA the citric acid productivity, Y ERY the citric acid yield
  4. The analysis was performed in triplicate. Cultures condition was described in the “Methods