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Table 1 Oxidation of various polysaccharide substrates by MtLPMO9A, MtLPMO9B and MtLPMO9C

From: Lytic polysaccharide monooxygenases from Myceliophthora thermophila C1 differ in substrate preference and reducing agent specificity

Substrate Occurrence of oxidation (upon addition of 1 mM ascorbic acid)
MtLPMO9Aa MtLPMO9B MtLPMO9C No enzyme
GlcOS # * bn XOS # * cn GlcOS #n XOS #n GlcOSn* XOSn* GlcOSn* XOSn*
Cellulose
 RACd + + + +
Hemicellulose
 Glucan
  Xyloglucane + +
  β-Glucan barleye +
  β-Glucan oat spelte + +
 Xylan
  OSXf
  BiWXf
  WAXf
Oligosaccharides
 Gluco-oligosaccharidesg
 Xylo-oligosaccharidesg
RAC/Hemicellulose combination
 RAC + BiWX + + + +
 RAC + OSX + + + +
 RAC + WAX + + +
  1. Oligosaccharides released and not released refers to + and −, respectively
  2. aData from Frommhagen et al. [ 2 ]
  3. bGluco-oligosaccharides oxidized at the C1 (GlcOS #n ) or C4 position (GlcOSn*)
  4. cXylo-oligosaccharides oxidized at the C1 (XOS #n ) or C4 position (XOSn*)
  5. dRegenerated amorphous cellulose (RAC)
  6. eXyloglucan from tamarind seed, β-(1 → 3, 1 → 4)-glucan from barley and β-(1 → 3, 1 → 4)-glucan from oat spelt
  7. fOat spelt xylan (OSX), birchwood xylan (BiWX), wheat arabinoxylan (WAX)
  8. gβ-(1 → 4)-linked gluco- and xylo-oligosaccharides, degree of polymerization 2–5