Skip to main content

Advertisement

Table 3 Yields of n-undecane of WT and some cADO mutants against n-dodecanal in the presence of the competition substrates

From: Structure-oriented substrate specificity engineering of aldehyde-deformylating oxygenase towards aldehydes carbon chain length

Mutants Yield of n-undecane (μM)
C12 C12/C7 C12/C18
WT 1.95 ± 0.13 1.55 ± 0.12 1.61 ± 0.064
V184F 8.65 ± 0.0.28 4.04 ± 0.35 9.75 ± 0.038
F87Y 3.01 ± 0.0043 1.74 ± 0.16 1.50 ± 0.0091
I27F 4.30 ± 0.13 1.91 ± 0.061 2.27 ± 0.11
V28Y 3.51 ± 0.12 1.78 ± 0.31 3.19 ± 0.39
  1. Yields of n-undecane of WT and some cADO mutants against n-dodecanal (150 μM) in the presence of the competition substrates (2 mM n-heptanal or 150 μM n-octadecanal) were determined by GC–MS