Skip to main content

Table 3 Yields of n-undecane of WT and some cADO mutants against n-dodecanal in the presence of the competition substrates

From: Structure-oriented substrate specificity engineering of aldehyde-deformylating oxygenase towards aldehydes carbon chain length

Mutants

Yield of n-undecane (μM)

C12

C12/C7

C12/C18

WT

1.95 ± 0.13

1.55 ± 0.12

1.61 ± 0.064

V184F

8.65 ± 0.0.28

4.04 ± 0.35

9.75 ± 0.038

F87Y

3.01 ± 0.0043

1.74 ± 0.16

1.50 ± 0.0091

I27F

4.30 ± 0.13

1.91 ± 0.061

2.27 ± 0.11

V28Y

3.51 ± 0.12

1.78 ± 0.31

3.19 ± 0.39

  1. Yields of n-undecane of WT and some cADO mutants against n-dodecanal (150 μM) in the presence of the competition substrates (2 mM n-heptanal or 150 μM n-octadecanal) were determined by GC–MS